鼻息肉中血管内皮生长因子和转化生长因子β_1的表达  

Expression of vascular endothelial growth factor and transforming growth factor-β_1 in nasal polyps

作　　者：郭剑锋[1] 王向东[1] 陶格陶呼 张呼和[1] 诸小侬 张伟 范尔钟 韩德民 

机构地区：[1]呼和浩特内蒙古医学院第一附属医院耳鼻咽喉科,010050 [2]北京耳鼻咽喉科研究所

出　　处：《中华耳鼻咽喉科杂志》2001年第2期83-86,T001,共5页Chinese Journal of Otorhinolaryngology

摘　　要：目的　探讨血管内皮生长因子 血管通透性因子 (vascularendothelialgrowthfactor vasularpermeabilityfactor,VEGF VPF)和转化生长因子β1 (transforminggrowthfactor β1 ,TGF β1 )在鼻息肉组织中的表达及意义。方法　 34例鼻息肉标本及 30例中鼻甲粘膜标本行VEGF VPF及TGF β1 的免疫组化染色 ,光镜观察。结果　①VEGF VPF在鼻息肉组织的血管内皮细胞和腺体细胞的表达明显高于中鼻甲组织 (P <0 .0 1和P <0 .0 5 ) ;②TGF β1 在鼻息肉组织的细胞外基质和固有层浸润细胞的表达明显高于中鼻甲组织 (P <0 .0 0 5 ) ;③鼻息肉组织中TGF β1 阳性细胞的形态及分布相似于嗜酸粒细胞 ;④VEGF VPF与TGF β1 阳性表达与鼻息肉的临床分型无关 (P >0 .0 5 )。结论　①VEGF VPF对鼻息肉发生过程中组织极度水肿的产生可能有非常重要的作用 ;②TGF β1 可能直接参与鼻息肉的病理变化 ,导致上皮基底膜增厚和基质纤维化 ;③嗜酸粒细胞可能为鼻息肉中TGF β1 的主要来源。Objective To study the expression and significance of vascular endothelial growth factor/vascular permeabilily factor (VEGF/VPF) and transforming growth factor β 1 (TGF β 1) in nasal polyps. Methods Expression of VEGF/VPF and TGF β 1 in nasal polyps from 34 patients and middle turbinates from 30 patients with deviation of nasal septum was prospectively studied with immunohistochemistry. Tissue sections were observed under optical microscope. Results (1)The VEGF/VPF positivity in vascular endothelium and in glandular cell was significantly higher in nasal polyps than in middle turbinates ( P <0.01 and P <0.05, respectively); (2)The TGF β 1 positivity in extracellular matrix and in cells in the stroma was significantly higher in nasal polyps than in middle turbinates( P <0.005); (3)The distribution and shape of TGF β 1 expressing cells in nasal polyps were similar to that of eosinophil, their positivities were significantly correlative; (4)The positivity of VEGF/VPF and TGF β 1 did not correlate with clinical type in nasal polyps ( P >0.05). Conclusion (1) The VEGF/VPF may play a key role in the formation of heavy edema of nasal polyps; (2) The TGF β 1 may contribute to some of the pathologic changes observed in nasal polyps,such as thickening of the epithelial basement membrane and stromal fibrosis; (3)Eosinophils in nasal polyps represent a major source of TGF β 1.

关 键 词：鼻息肉 血管内皮生长因子 转化生长因子Β 免疫组织化学 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]