4-氨基吡啶对牛磺酸调节突触体氨基酸释放的影响  

EFFECT OF 4-AMINOPYRIDINE ON TAURINE-REGULATED RELEASE OF AMINO ACID FROM RAT CORTICAL SYNAPTOSOMES

作　　者：郑里翔[1] 

机构地区：[1]江西中医学院,江西南昌330006

出　　处：《药学学报》2001年第4期307-309,共3页Acta Pharmaceutica Sinica

基　　金：江西省青年基金资助项目! (986 40 76 )

摘　　要：AIM To elucidate the mechanism of taurine-regulated amino acid release from synaptosomes. METHODS Endogenous aspartate, glutamate and GABA release from cortical synaptosomes were measured by high performance liquid chromatography using stepwise elution system, Glutamate release was monitored by continuous fluorometry. RESULTS 4-Aminopyridine (3 0×10 -2 mol·L -1 ) counteracted the taurine-induced inhibition of glutamate overflow ( P <0 05), while aspartate and GABA release was not affected. Nimodipine (10 -5 mol·L -1 ) combined with 4-aminopyridine was shown to decrease glutamate release ( P <0 05). CONCLUSION Taurine may regulate glutamate release through presynaptic L -type calcium channel and aslo act on Asp-and GABA-nereve terminal to regulate Asp and GABA release in rat cortex.AIM To elucidate the mechanism of taurine-regulated amino acid release from synaptosomes. METHODS Endogenous aspartate, glutamate and GABA release from cortical synaptosomes were measured by high performance liquid chromatography using stepwise elution system, Glutamate release was monitored by continuous fluorometry. RESULTS 4-Aminopyridine (3 0×10 -2 mol·L -1 ) counteracted the taurine-induced inhibition of glutamate overflow ( P <0 05), while aspartate and GABA release was not affected. Nimodipine (10 -5 mol·L -1 ) combined with 4-aminopyridine was shown to decrease glutamate release ( P <0 05). CONCLUSION Taurine may regulate glutamate release through presynaptic L -type calcium channel and aslo act on Asp-and GABA-nereve terminal to regulate Asp and GABA release in rat cortex.

关 键 词：4-氨基吡啶 牛横酸 突触体 谷氨酸 天冬氨酸 Γ-氨基丁酸 尼莫地平 

分 类 号：R338[医药卫生—人体生理学] R977.4[医药卫生—基础医学]