豚鼠心脏表面神经节丛SP、CGRP及VIP的免疫组织化学研究  

Immunohistochemical study on SP,CGRP and VIP in superficial ganglial plexus of the guinea pig heart

作　　者：马东亮[1] 李月英[1] 王唯析[1] 胡海涛[1] 赵志英[2] 袁秉祥[3] 

机构地区：[1]西安交通大学医学院人体解剖学教研室,,西安710061 [2]内蒙古包头医学院解剖学教研室 [3]西安交通大学医学院药理学教研室

出　　处：《西安医科大学学报》2001年第1期11-13,共3页Journal of Xi'an Medical University(Chinese)

基　　金：国家自然科学基金!资助项目 (No .396 70 2 45 )

摘　　要：目的　研究SP、CGRP及VIP在心脏表面 3个主要神经节丛 ,右心房神经节丛 (RAGP)、心房背侧神经节丛 (DAGP)及主动脉与肺动脉间神经节丛 (A PGP)的化学性质和定位分布。方法　应用免疫组织化学ABC法 ,对豚鼠心脏表面 3个主要神经节丛的神经元及神经纤维进行了SP、CGRP及VIP免疫组织化学染色及光镜观察。结果　在上述三个神经节丛内 ,未发现SP及CGRP免疫反应阳性神经元 ,但可见少量SP免疫反应阳性神经纤维分布 ,CGRP免疫反应阳性神经纤维数量较SP阳性纤维密集 ,尤其在A PGP更为明显 ;VIP免疫反应阳性神经元和神经纤维分布最为密集 ,交织成神经节丛。结论　豚鼠心脏表面主要神经节丛内确实存在SP、CGRP及VIP ,并且这 3种神经肽可能直接参与心肌细胞和心脏血管活动的调控。Objective To investigate the distribution and the chemical properties of three predominant superfical ganglial plexus which are the right atrial ganglial plexus(RAGP),the dorsal atrial ganglial plexus(DAGP) and the ganglial plexus between aorta and pulmonary artery(A PGP) in the heart.Methods The study was performed concering the neurons and fibers of SP,CGRP and VIP immunoreactive(IR) substance in three predominant superfical ganglial plexus of the guinea pig heart by immunohistochemical ABC method and observed by light microscope.Results The SP IR and CGRP IR neurons could not be found in the above mentioned ganglial plexus.But SP IR fibers distributed sparsely,CGRP IR fibers were more densely than SP IR fibers,especially A PGP.The population of VIP IR neurons and fibers were most dense and interweaved ganglial plexus.Conclusion There were actually SP,CGRP and VIP in three predominant superfical ganglial plexus of the guinea pig heart and the three peptides could possibly modify the activities of myocardiocytes and vessels of heart directly.

关 键 词：豚鼠 心脏 神经节丛 P物质 SP 降钙素基因相关肽 CGRP 血管活性肠肽 VIP 免疫组织化学 

分 类 号：R541.02[医药卫生—心血管疾病] R322.85[医药卫生—内科学]