大鼠蓝斑酪氨酸羟化酶样神经元至背海马投射观察  

The tyrosine hydroxylasic projections from locus coeruleus to the dorsal hippocampus in rats

作　　者：王建祯[1] 马朝显 崔建军[3] 周明付[4] 张华[4] 林玲[4] 汤善钧[4] 

机构地区：[1]河南医科大学第二附属医院神经外科,郑州450003 [2]中原油田总医院第二分院外科,濮阳457000 [3]焦作市第二人民医院神经外科,焦作454001 [4]河南医科大学人体解剖学教研室,郑州450052

出　　处：《河南医科大学学报》2001年第2期174-176,共3页Journal of Henan Medical University

摘　　要：目的 :观察大鼠蓝斑至背海马投射纤维的递质性质。方法 :用辣根过氧化物酶 (HRP)逆行追踪法和免疫组织化学相结合的双重标记技术研究蓝斑TH样神经元至背海马投射。结果 :在双侧蓝斑内可见到HRP标记细胞、TH阳性反应细胞和TH HRP双标细胞 ,同侧明显多于对侧。结论 :背海马接受双侧蓝斑纤维的投射 。Aim: To demonstrate neurotransmitters in fibers projeced to dorsal hippocampus from locus coeruleus in rats. Methods: Using the double labelling technique which combines the immunocytochemical Avidin-Biotin-Peroxidase complex (ABC)method with the Horseradish Peroxidase (HRP)retrograde tracing method to study the neurotransmitters in fibers projected to dorsal hippocampus from locus coeruleus. Results: HRP labelled cells,TH labelled cells and TH-HRP double labelled cells were found in locus coeruleus. Conclusion: The data of the projection is of some value in further study on the physiological function of hippocampus and the embryonic transplantation including hippocampus.

关 键 词：背海马 蓝斑 酪氨酸羟化酶 免疫组织化学 大鼠 辣根过氧化物酶 神经元 

分 类 号：R338.26[医药卫生—人体生理学]