骨器官体外培养的方法学研究  

Study on the method of organ culture of bone in vitro

作　　者：刘开泰[1] 马丽英[2] 李灵芝[3] 王瑞淑[2] 王国荃[1] 汪师贞[1] 

机构地区：[1]新疆医科大学公共卫生学院,830054 [2]华西医科大学公共卫生学院,四川成都610041 [3]天津武警医学院,天津300070

出　　处：《新疆医科大学学报》2001年第1期8-10,共3页Journal of Xinjiang Medical University

摘　　要：目的 :探讨长骨器官体外培养的最佳条件。 方法 :游离受孕第 16天小鼠胚胎尺骨在体外进行培养 ,观察培养基 p H值、血清含量和通气方式等因素对尺骨生长发育的影响。结果 :将尺骨置于 p H为 7.2～ 7.4、血清含量为 10 %～ 2 0 %的 BGJ( b)培养基 ,以连续通气或每 2 4小时通气一次的方式进行体外培养 ,可保证培养尺骨正常生长。 结论 :本研究为探索有害物质 (如氟等 )Objective: To investigate the optimum of organ culture of long bone in vitro. Method: By means of the method of autorotation with constant temperature, ulnas from 16 day old mouse embryos were cultured in vitro. Results: The growth and development of bone were better under condition of pH 7.2～7.4, 10%～20% serum in the culture medium and continuous (or once a day) supplementation of gas mixture. Conclusion: This study provided a useful experimental condition for investigating the skeletal damage of toxicants.

关 键 词：骨器官培养 培养基pH 培养基血清含量 通气方式 骨骼损伤 小鼠 

分 类 号：R681[医药卫生—骨科学] R－332[医药卫生—外科学]