定量ELISA检测小鼠血清中抗LDH-C4IgG2b抗体的水平  

Detection of IgG2b Monoclonal Antibodies Against LDH-C4 in Sera of Mice Bearing Hybridoma Backpack Tumors by Quantitative ELISA

作　　者：孙玲[1] 许金菊[1] 黄生民[1] 王晓蕾[1] 张永振[1] 曹筱梅[1] 贲昆龙[1] 

机构地区：[1]中国科学院昆明动物研究所,昆明650223

出　　处：《昆明医学院学报》2001年第1期30-33,共4页Journal of Kunming Medical College

基　　金：美国CONRAD基金!资助 (MFG96 - 15 )

摘　　要：用定量ELISA的方法检测背部携带分泌抗LDH -C4IgG2b抗体杂交瘤小鼠的血清中IgG2b抗体浓度 ,结果表明 :本法稳定 ,批间变异系数为 7 0 4%～ 13 30 % ,批内变异系数为 3 6 1%～ 10 2 0 % ;标准曲线相关性良好 ,相关系数从 0 96 2 884～ 0 996 795 ;灵敏度较高 ,最低检出浓度为 0 0 1mg/L ;The levels of IgG2b monoclonal antibodies against LDH-C4 in sera of mice bearing hybridoma backpack tumors secreting anti-LDH-C4-IgG2b were detected by quantitative ELISA. The accuracy between batches is 7 04%～l3 30%, the intra-assay variation is 3 6l%～l0 20%. Standard curveof monoclonal lgG2b was well correlated ( r =0 962884～0 996795). The sensitivity of the assay reache dup to0 0l?mg/L. The present modified ELISA offers a reproducible, sensitive, specific method in determination of antigen-specific IgG2b antibody in sera.

关 键 词：定量ELISA LDH-C4 IgG2b 乳酸脱氢酶-C4 血清诊断 单克隆抗体 抗体 

分 类 号：R446.62[医药卫生—诊断学]