吲哚美辛和美洛昔康对脂多糖诱导的小鼠腹腔巨噬细胞NF-κB的抑制作用  

INHIBITORY EFFECTS OF INDOMETHACIN AND MELOXICAM ON NF-κB IN MOUSE PERITONEAL MACROPHAGES

作　　者：胡玉芳[1] 郭颖[1] 程桂芳[1] 

机构地区：[1]中国医学科学院,中国协和医科大学药物研究所,北京100050

出　　处：《药学学报》2001年第3期161-164,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金! (39870 90 7)

摘　　要：目的　研究非甾体抗炎药吲哚美辛 (indomethacin)和美洛昔康 (meloxicam)对细菌脂多糖 (LPS)诱导表达的C5 7小鼠腹腔巨噬细胞核转录因子NF κappaB(NF κB)的抑制作用。 方法　NF κB的测定采用电泳迁移率改变法。结果　小鼠腹腔巨噬细胞经LPS诱导后细胞NF κB含量明显增高。Indomethacin和meloxicam在 10 -5- 10 -7mol·L-1浓度下可明显降低LPS激活的小鼠腹腔巨噬细胞NF κB活化。结论　Indomethacin和meloxicam对NF κB活化的抑制作用可能是两者的抗炎作用机理之一。AIM To study the inhibitory effects of indomethacin and meloxicam on NF κB from lipopolysaccharide (LPS) stimulated peritoneal macrophages of mice. METHODS NF κB was measured with the method of electrophoretic mobility shift assay (EMSA). RESULTS After induction by LPS at the concentrations of 1 and 3 μg·mL -1 , the NF κB content of the mouse peritoneal macrophages increased markedly. Indomethacin and meloxicam, at the concentrations of 10 -7 -10 -5 mol·L -1 , decreased the activation of NF κB at the concentrations of 1 and 3 μg·mL -1 in activated mouse peritoneal macrophages induced with LPS at the concentrations of 1 and 3 μg·mL -1 . CONCLUSION The inhibitory effects of indomethacin and meloxicam on NF κB activation may be one of their mechanisms of antiinflammatory actions.

关 键 词：细胞核转录因子 吲哚美辛 美洛昔康 电泳迁移率改变法 环氧酶2抑制剂 脂多糖 非甾体抗炎药 

分 类 号：R971.1[医药卫生—药品]