系统性红斑狼疮患者可溶性肿瘤坏死因子受体及肿瘤坏死因子α水平的研究  

Levels of sTNFR、 TNFα in Serum and PBMC Culture Medium Supernatants of Systemic Lupus Erythematosus

作　　者：张开明[1] 杨小英[1] 尹兴平[1] 唱文娟[1] 李慧仙[1] 

机构地区：[1]山西省太原市中心医院皮肤科,030009

出　　处：《中华皮肤科杂志》2001年第1期31-33,共3页Chinese Journal of Dermatology

摘　　要：目的了解系统性红斑狼疮（ SLE）患者血清中可溶性肿瘤坏死因子受体 (sTNFR)和肿瘤坏死因子α (TNFα )水平、两者的比值以及同临床症状的关系。方法采用双抗体夹心 ELISA法检测血清和外周血单一核细胞（ PBMC）培养上清液中 TNFα、 sTNFRⅠ及 sTNFRⅡ水平，同时检测其它免疫项目。结果① SLE患者血清中 sTNFRⅠ、 sTNFRⅡ、 TNFα水平及 sTNFRⅠ /TNFα和 sTNFRⅡ /TNFα比值显著高于正常人对照组 (P< 0.01), sTNFRⅠ与 sTNFRⅡ之间呈显著正相关 (r=0.88, P< 0.01)。② PBMC培养上清液中 TNFα、 sTNFRⅠ及 sTNFRⅡ水平显著高于正常人对照组，且与血清中的 TNFα和 sTNFR浓度呈显著正相关（ r=0.58～ 0.73;P< 0.01）。③ TNFα与感染有平行关系，而 sTNFRⅠ和 sTNFRⅡ同临床症状评分呈正相关 (r值分别为 0.69和 0.75)。结论 SLE患者 sTNFR、 TNFα水平及 sTNFR/TNFα均有异常， sTNFRⅠ对封闭和抑制 TNFα起作用，而 sTNFRⅡ可能在协助 sTNFRⅠ及炎症方面发挥作用， sTNFRⅠ及 sTNFRⅡ对于监测 SLE疾病活动和严重程度明显优于抗 dsDNA抗体等指标。Objective To study the levels of soluble tumor necrosis factor receptor (sTNFR)、 tumor necrosis factor alpha(TNFα ) in systemic lupus erythematosus(SLE) patients and its clinical significance. Methods Double antibody sandwich ELISA was used to determine soluble tumor necrosis factor receptor typeⅠ (sTNFRⅠ )、 soluble tumor necrosis factor receptor typeⅡ (sTNFRⅡ ) and TNFα levels in patient serum and peripheral blood mononuclear cell(PBMC) culture supernatants. Other immunological tests were carried out simultaneously. Results① The serum sTNFRⅠ、 sTNFRⅡ、 TNFα sTNFRⅠ /TNFα and sTNFRⅡ /TNFα ratios were significantly higher in SLE patients than those in normal control group(P< 0.001).There was a significant positive correlation between the levels of sTNFRⅠ and sTNFRⅡ (r=0.88;P< 0.001).② The levels of TNFα、 sTNFRⅠ and sTNFRⅡ in SLE PBMC culture supernatant were significantly higher than those of normal controls with a positive correlation to the serum levels(r=0.58～ 0.73;P< 0.001).③ The increased level of TNFα was related to infection,whereas the sTNFRⅠ、Ⅱ levels were positively correlated with the clinical scores. Conclusion There is an abnormal TNFα and sTNFR levels in SLE patients, which may play certain roles in the pathogenesis of SLE, and can be used as indices for active SLE.

关 键 词：系统性红斑狼疮 可溶性肿瘤坏死因子受体 肿瘤坏死因子 STNFR 

分 类 号：R593.241[医药卫生—内科学]