小鼠小肠上皮内淋巴细胞的提取与抗原表型测定和功能的初步研究  

Studies on isolation phonotype and natural cytotoxicity of murine intraepithelial lymphocytes

作　　者：周正任[1] 叶晓卉[1] 杨晓临[1] 王丹[1] 

机构地区：[1]中国医科大学病,原生物学教研室沈阳110001

出　　处：《中国免疫学杂志》2001年第3期135-137,共3页Chinese Journal of Immunology

基　　金：辽宁省自然科学基金资助! ( 96 2 316 )

摘　　要：目的 :建立一种提取小肠上皮内淋巴细胞的方法 ,并对其功能进行初步研究。方法 :采用机械分离法和Percoll非连续密度梯度离心法提取小鼠小肠上皮内淋巴细胞 ,经间接荧光染色后用流式细胞仪检测表型。应用乳酸脱氢酶法检测其自然杀伤功能。结果 :应用该法可获得小鼠小肠上皮内淋巴细胞 2× 10 6 ～ 4× 10 6 只鼠 ,活力测定 >95 %。抗原表型中73 8%以上表达CD8分子 ,TCRγδ为 43 8%。而且自然杀伤活性明显高于小鼠脾淋巴细胞。结论 :表明采用该法提取小鼠小肠上皮内淋巴细胞有效。Objective:To extract a method for isolating murine intraepithelial lymphocyte and analyze its main function.Methods:Murine intestinal intraepithelial lymphocyte was isolated by a mechanical technique and Percoll discontinous density gradient centrigugation.Its surface antigens were measured by using indirect immunoflurescence.Its natural killing activity was examined by LDH method.Results:2×10 6～4×10 6iIELs/per mouse were acquired by using this method,add its vitality>95%.Of phenotypic antigen,more than 73.8% showed CD 8 +cell,TCRγδ 43.8%.IEL's natural killing activity was higher than PBMCs.Conclusion:This method is effective of isolating IELs.IELs has natural killing activity.

关 键 词：小肠上皮内淋巴细胞 自然杀伤活性 抗原表型 小鼠 

分 类 号：R392.12[医药卫生—免疫学]