用柱前衍生反相高效液相色谱法测定L-磺基丙氨酸  

Determination of L-Cysteic Acid by Reversed-phase High-performance Liquid Chromatography

作　　者：赵建玲[1] 朱智甲[2] 蒋殿录[3] 

机构地区：[1]南阳师范学院化学系,河南南阳473061 [2]河北师范大学实验中心,河北石家庄050016 [3]河北师范大学化学系,河北石家庄050016

出　　处：《分析测试学报》2000年第5期72-74,共3页Journal of Instrumental Analysis

基　　金：河北省科委资助项目!(99213085D)

摘　　要：建立了一种测定L_磺基丙氨酸的反相高效液相色谱法 ,以邻苯二甲醛和巯基乙醇为柱前衍生化试剂 ,控制衍生化反应时间为2min ,固定相为Hypersil -C18(4.6mm×250mm,5μm) ,流动相为0.05mol/LNaAc -THF -CH3OH(体积比为30∶0.15∶70),荧光检测激发波长315nm ,发射波长450nm ,L_磺基丙氨酸在0～8.0μg间与峰面积呈现良好的线性关系 ,相关系数为0.9984 ,相对标准偏差为3.4 % (n=6,m=4.0μg)。A method for the determination of L _ cysteic acid by reversed _ phase high _ performance liquid chromatography was developed. A L _ cysteic acid sample was derivatized by o _ phthalaldehyde and mercaptoethanol for 2 min on pre _ column, then separated with Hypersil-C18 column and detected with a fluorescence detector. A 0.05 mol/L NaAc-CH3OH-THF(30∶70∶0.15 by volumn) system was used as a mobile phase with a flow rate 1.0 mL/min. There is a good linear relationship between content of L _ cysteic acid and the peak area in the range of 0～8.0 μg, with correlation coefficient r=0.998 4. The RSD of the method is 3.4%(n=6, m=4.0 μg). The method is simple, rapid and accurate.

关 键 词：L-磺基丙氨酸 反相高效液相色谱法 柱前衍生法 药物分析 

分 类 号：TQ464.7[化学工程—制药化工] R977.4[医药卫生—药品]