丝组二肽对DNA的切割作用的研究  

Studies on DNA Cleaved by Seryl-histidine Dipeptide

作　　者：万荣[1] 王宁[1] 赵玉芬[1] 

机构地区：[1]清华大学生命科学与工程研究院化学系教育部生命有机磷化学重点实验室,北京100084

出　　处：《高等学校化学学报》2001年第4期598-600,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金! (批准号 :2 963 2 0 0 4)资助

摘　　要：Linear and supercoiled DNA were cleaved by HPLC purified seryl histidine dipeptide(SH). It was found that the DNA fragments produced by the reaction of SH and DNA could be ligated together by T 4 DNA ligase. This result implied that the SH was the first example of the ion free artificial DNA cleavage agent that could split DNA by hydrolysis mechanism.Linear and supercoiled DNA were cleaved by HPLC purified seryl histidine dipeptide(SH). It was found that the DNA fragments produced by the reaction of SH and DNA could be ligated together by T 4 DNA ligase. This result implied that the SH was the first example of the ion free artificial DNA cleavage agent that could split DNA by hydrolysis mechanism.

关 键 词：丝组二肽 DNA 切割 水解机理 人工核酸切割试剂 

分 类 号：Q523[生物学—生物化学]