结晶紫共振光散射法测定脱氧核糖核酸  

Determination of Deoxyribonucleic Acid with Crystal Violet by Resonance Light Scattering Method

作　　者：刘晨[1] 胡灏[1] 陈小明[1] 

机构地区：[1]湘潭大学化学化工学院,湘潭411105

出　　处：《分析试验室》2001年第2期30-33,共4页Chinese Journal of Analysis Laboratory

基　　金：湖南省自然科学基金!项目 ( 0 0 JJY2 0 84 )

摘　　要：研究了三苯甲烷类碱性染料结晶紫与 DNA作用的共振光散射光谱 ,在p H 9.2～ 1 0 .5的范围内 ,加入 DNA导致结晶紫共振光散射增强 ,在 51 2 nm处 ,存在一共振光散射增强峰 ,其强度与 DNA的浓度呈线性关系 ,据此建立了一种测定 DNA的共振光散射法。方法的线性范围为 0～ 90 0 ng/m L,检出限为5.0 2 ng/m L。已用于混合样品中 DNA的测定。Resonance light scattering (RLS) spectra of crystal violet with DNA was studied. The RLS of crystal violet was greatly enhanced by DNA in the range of pH 9.2～10.5. A resonance light scattering peak at 512nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0～900 ng/mL with the detection limit of 5.02 ng/mL. Compared with other methods, this method has larger detective wavelength, which can reduce the interference of background and increase the intensity of resonance light scattering.

关 键 词：结晶紫 共振光散射法 DNA 测定方法 

分 类 号：Q523.03[生物学—生物化学]