硝酸铈对钙调素及调节蛋白Ca^(2+)-ATP酶基因表达的影响  

Effect of Cerium Nitrate on CaMⅠ, PMCA1b and mRNA Levels

作　　者：杨维东[1] 刘洁生[1] 王艇[2] 雷衡毅[3] 杨燕生[3] 

机构地区：[1]暨南大学生物工程系,广东广州510632 [2]中山大学生命科学学院,广东广州510275 [3]中山大学化学化工学院

出　　处：《中国稀土学报》2001年第1期71-74,共4页Journal of the Chinese Society of Rare Earths

基　　金：国家自然科学基金! (2 96 710 34);广东省自然科学基金! (980 32 9)资助项目

摘　　要：运用逆转录聚合酶链式反应研究了硝酸铈对大白鼠肝脏CaMⅠ ,PMCA1b基因表达的影响。腹腔注射高、低浓度硝酸铈均不会引起CaMⅠ ,PMCA1b基因表达的变化 ,提示硝酸铈对钙调素、Ca2 + ATP酶的影响可能仅处于转录后水平。The effect of cerium nitrate on expression of CaMⅠ, PMCA1b and mRNA in rat hepatocyte was studied by means of reverse transcription polymerase chain reaction. The results show that no remarkable increase in expression of CaMⅠ, PMCA1b and mRNA in hepatic tissue occurs after recurrent cerium nitrate administered intraperitoneal (1, 50 mg·kg -1 ), which suggests that the effect of rare earth on CaM and Ca 2+ ATPase might be at posttranscription level.

关 键 词：硝酸铈 钙调素 逆转录聚合酶链式反应 钙(Ⅱ)-ATP酶 基因表达 肝脏 

分 类 号：R972[医药卫生—药品] Q556[医药卫生—药学]