rhIL-11不同时间给药对急性放射病猕猴造血系统的影响  

Therapeutic effect of rhIL-11 administered at different times on acute radiation sickness in rhesus monkeys

作　　者：郝静[1] 罗庆良[1] 熊国林[1] 柳晓兰[1] 邱丽玲[1] 陈国志[1] 黄缨[1] 葛平[1] 耿煜[1] 孙连生[1] 董波[1] 李元敏[1] 陈望秋 沈纯 

机构地区：[1]北京放射医学研究所,100850 [2]医进生物技术(北京)有限公司

出　　处：《中华放射医学与防护杂志》2001年第1期31-34,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：全军"九五"指令性课题!基金资助项目 (96 0 5 0 0 1)

摘　　要：目的　观察rhIL 11不同时间给药对60 Coγ射线照射猕猴造血系统的影响。方法　正常成年猕猴用60 Coγ射线一次全身照射 3 .0Gy ,吸收剂量率 15 4.96cGy/min ,分照射对照、早期给药和后给药 3组 ,后两组分别在照射后当天和第 13天开始皮下注射rhIL 116 0 μg·kg-1·d-1(μKD) ,每日 1次 ,连续 14d。 结果　照射后应用rhIL 11可明显缩短照射猴外周血小板数和白细胞数最低值的持续时间 ,早期给药还可明显升高照射猴外周血小板数的最低值。给药后 1周内 ,早期给药组外周血红细胞数较照射对照组有一定程度的降低 ,从给药后第 19天开始则明显高于照射对照组。照射后 45d骨髓细胞培养结果表明 ,rhIL 11可明显刺激照射猴骨髓有核细胞体外形成CFU MK和CFU Mix的能力。病理组织学观察结果同样表明rhIL 11治疗组骨髓粒系和巨核系细胞明显增殖。结论　rhIL 11可明显促进急性放射病猴骨髓造血功能的恢复 ,照射后早期给药的效果优于第 13天开始给药。Objective\ This report describes the therapeutic effect of recombinant human interlukine\|11(rhIL\|11)administered at different times on acute radiation sickness in monkeys. Methods Rhesus monkeys irradiated with 3.0 Gy 60 Co γ\|rays were divided into 3 groups.One group was the control administered with vehicle,the second one was subjected to administer rhIL\|11 on days 0\|13 after TBI(60 μg·kg -1 ·d -1 ,sc) and the third one to administer rhIL\|11 on days 13-26 after TBI at the same doses. Results\ The early treated group had higher platelet nadirs compared with that of the other two.The duration of platelet and leukocyte numbers below 50% of their baseline values shortened significantly in animals treated with rhIL\|11,especially in the early treated group.During the first week after irradiation,the early treated group had lower erythrocyte count compared with the control,but it began to rise at day 19 after irradiation.Semi\|solid bone marrow cell culture demonstrated that rhIL\|11 could stimulate bone marrow cells to form more CFU\|MK,CFU\|Mix,CFU\|E,BFU\|E and CFU\|GM in vitro .We also got the same results in histopathological observation. Conclusion\ rhIL\|11 administered at different times can not only accelerate the haemotopoietic recovery of acute radiation sickness in rhesus monkeys,but also result in better therapeutic effect when administered earlier.

关 键 词：急性放射病 RHIL-11 造血细胞生长因子 

分 类 号：X591[环境科学与工程—环境工程]