光镜和电镜研究免疫酶的组织化学染色法  

IM AND EM STUDIES OF HTSTOCHEMICAL STAINING METHOD FOR THE IMMUNOENZYME

作　　者：高俊峰[1] 名仓宏[2] 

机构地区：[1]中国医科大学第二电子显微镜室 [2]名古屋大学医学部生体防御研究室

出　　处：《中国医科大学学报》1990年第1期29-32,共4页Journal of China Medical University

摘　　要：本文对免疫酶组织化学的样品制备程序和染色方法做了详细的阐述。用直接法、间进法和ABC法,对IgA、Leu4、HLA-DR、CEA和4型角蛋白的定位,进行了光镜和电镜的观察,染色阳性反应显著,获得了满意的效果。并对染色技巧做了分析和探讨。In this article, the precedures for histochemical sample preparation ofimmunoenzyme and its staining method are elaborated. With direct, indirectand ABC methods, the local distribution of IgA, Leu4, HLA-DR, CEA andtype 4 keratin in human intestinal mucosa was observed under LM and EM.The positive reaction of staining was remarkable with satisfactory results. The skill of staining is also analysed and discussed.

关 键 词：免疫组织化学 辣根过氧化物酶 二氨基联苯胺 免疫酶 光镜 电镜 

分 类 号：R392.1[医药卫生—免疫学]