外源性表皮生长因子在庆大霉素所致严重急性肾小管坏死中的作用  

Effect of exogenous administration of epi-dermal growth factor in the recovery phaseof gentamycin-induced acute tuhular necro-sis

作　　者：于阳[1] 郑法雷[1] 薄玉红[1] 毕增祺[1] 黄华良 黄庆元 卜玉芬 

机构地区：[1]中国协和医科大学协和医院肾内科,中国科学院遗传所

出　　处：《肾脏病与透析肾移植杂志》1995年第1期18-20,共3页Chinese Journal of Nephrology,Dialysis & Transplantation

摘　　要：本实验观察了外源性ＥＧＦ在庆大霉素（ＧＭ）引起的重度ＡＴＮ修复期的作用。实验动物分为三组：①ＧＭ致ＡＴＮ组（６组）；②ＡＴＮ＋ＥＧＦ治疗组（Ｇ＋Ｅ组）；③正常对照组（ＮＬ组）．分别在ＧＭ或ＧＭ＋ＥＧＦ注射后０，１，３，５，８天测定肾小管上皮细胞的３ＨＴｄＲ掺入率和血清肌酐。结果示：Ｇ＋Ｅ组动物肾小管上皮细胞的３ＨＴｄＲ掺入率比Ｇ组动物明显增加，但Ｇ＋Ｅ组的肾功能和病理表现较Ｇ组无明显改善。本研究结果表明外源住ＥＧＦ能促进ＧＭ中毒性ＡＴＮ肾小管上皮细胞的再生和ＤＮＡ合成，但其对动物肾功能恢复无明显作用，其原因尚待进一步探讨。t is suggested that some growth fac-tors may play important roles in repairingand recovery of acute tubular necrosis(ATP). To examine the effect of exogenousadministration of EGF in the recovery phaseof gentamycin (GM)-induced ATN, ratswere divided into three groups : ①GM-treat-ed only (G, n = 21 ) , rats were adminis-tratered GM 400 mg/kg, 3 days ip; ②GMand EGF-treated ( G + E , n = 2 5 ) , EGF ( 25μg) was injected subcutaneously 1 h afterthe final GM injection;③Normal rats (NL,n= 7) ,rats were not treated . 3H thymidineincorporation into renal tissue and serumcreatininc concentration were measured atvarious times after GM administration. Thelevels of renal 3H thymidine incorporation ingroup G+E were siginificantly higher thanthat in group G at different time intervalsafter toxic injury(at day 1 ,85. 5± 14. 94 vs.57. 6±19. 71 cpm/μg DNA ,P<0. 05 ;at day5 , 98. 0± 9. 54 vs 27. 3 ± 1. 53 cpm/μgDNA,P <0. 001 ). However . serum creatinine ley-els were not different between group G andG + E. These findings indicate that exoge-nous EGF may accelerate the DNA synthe-sis in renal tubule cells in GM-inducedATN, but their effect on improvement ofkidney function in GM-induced ATN needsto be further studied.

关 键 词：表皮生长因子 急性 肾小管坏死 恢复 

分 类 号：R983[医药卫生—药品] R692.605[医药卫生—药学]