流行性出血热患者活检肝脏的抗原定位和超微病理研究  

STUDY OF ANTIGEN LOCATION AND ULTRAMICROSCOPIC PATHOLOGY IN LIVER BIOPSIES FROM EHF PATIENTS

作　　者：张家驹[1] 陈卫宁[1] 杜景云[1] 张忠玲[1] 洪涛[2] 盛鹏 吴世英[3] 张作卿[3] 郭超英[3] 

机构地区：[1]山东省医学科学院 [2]中国预防医学科学院病毒学研究所 [3]济南市传染病医院

出　　处：《山东医科大学学报》1991年第4期271-274,共4页Acta Academiae Medicinae Shandong

摘　　要：采用免疫胶体金标记技术和低温包埋与常规电镜观察相结合的方法,研究了EHF患者活检肝脏标本,首次发现肝细胞内的EHF病毒,证实病毒可侵袭肝实质细胞。在早期患者肝狄氏间隙内见到Ag-Ab复合物,认为早期患者体内也存在免疫复合物反应。同时发现以肝细胞膜、内质网、线粒体等膜性结构损伤为特征的细胞损害,证明了病毒对肝脏的直接损伤作用。由此对EHF的发病机理提出了新的学说。Liver biopsies used in this study were examined with the im-proved method of immunocollodial gold-labelling,the low temperature embe-dding medium and routine electron microscope examination。We found EHF virus in the patient′s liver cells.This discovery indicated that EHF virus is not only involved in the Kuffer′s cell and artery endotheliocyte but also invades and attacks the parenchyma(liver cells)。The ultrastructures of the EHF patient′s liver cells were studied.We found that damage of the cell membrane and injury of mitochondria endoplasmic reticutum were pathologically characteristic.These results indicated that EHF virus could directly damage the liver parenchyma.In the early stage of the disease antigen-antibody complexes in the Disse′s spaces could be seen in biopsy specimens.This discover may explain the pathogenesis of EHF in the early stages of the disease。It resembled the type Ⅲ allergy reaction.According to these results,this could be a new direction for the EHF research.This hypothesis would also have some significance on the basic research on treatmett and prevention of EHF.

关 键 词：流行性出血热 活组织检查 抗原 免疫测定 显微镜检查 电子 低温包埋 

分 类 号：R512.8[医药卫生—内科学]