水稻齿叶矮缩病毒在水稻病叶及传毒媒介昆虫组织内的形态  

RICE RAGGED STUNT VIRUS IN THE PHLOEM CELLS OF DISEASED RICE AND IN THE SALIVARY GLAND CELLS OF INFECTED VECTOR

作　　者：沈菊英[1] 彭宝珍[1] 龚祖埙[1] 林瑞芬[2] 金登迪[2] 

机构地区：[1]中国科学院上海生物化学研究所 [2]浙江农业科学院病毒研究室

出　　处：《上海农业学报》1989年第2期15-18,共4页Acta Agriculturae Shanghai

基　　金：国家自然科学基金资助课题

摘　　要：用饲毒后的褐稻虱进行单虫单苗接种,稻苗发病率达60％以上。对病株的叶脉脉肿部位及相应编号的褐稻虱唾液腺进行超薄切片后,在电子显微镜下观察,病株的筛管细胞和传毒昆虫的唾液腺细胞内可见到大量病毒颗粒,并以晶状排列或者包裹于管状组织中。病毒颗粒大小约为70nm,具有45nm致密核心为双链RNA的组成部分,在核酸和核衣壳之间尚有空隙。本文对病毒的细微结构进行了讨论,并提供进一步证据,说明我国发现的RRSV和国外报道起源于东南亚的RRSV是同一种病毒。The rice brown planthopper (Nilaparvata Lugens) has been used for transmission assays of Rice Ragged Stunt-Virus (RRSV). About 60% of hoppers can transmit the virus. Electron microscopic observations of thin sections of phloem cells of the enations of diseased rice leaves and of salivary gland cells of infected vectors revealed the massive virions in both cells. The virion contains dense RNA core and coat protein layer with the size and width of 45 nm and 10—12 nm,respectively. Between the core and layer a gap with 12—15 nm can be clearly seen. Virus particle-containing tubules are found in infected vector cells but have not been fouud in diseased plant cells. In the latter case the virion are embedded in fibrillar viroplasma.

关 键 词：水稻 齿叶矮缩病毒 病毒形态 寄主细胞超薄切片 褐稻虱 传毒媒介昆虫组织 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] S432.41[农业科学—植物保护]