基因枪法转化水稻(Oryza sativaL.)获得可育的转抗虫基因水稻再生植株  

Obtaining Fertile Transgenic Plants of Resistant insect Rice (Oryza sativa L.) via Particle Bombardment

作　　者：安韩冰[1] 朱祯[2] 李慧芬[2] 吴茜[2] 周兆澜[2] 徐鸿林[2] 徐军望[2] 安利佳[1] 李向辉[2] 

机构地区：[1]辽宁师范大学生物系,大连116022 [2]中国科学院遗传研究所,北京100101

出　　处：《高技术通讯》2001年第2期12-16,11,共6页Chinese High Technology Letters

基　　金：86 3计划资助项目!(10 1 0 1 0 1 0 1);86 3计划生物技术领域中试研究项目;国家转基因植物研究与产业化开发专项基金!(ZJY A 0 7)

摘　　要：应用基因枪 ( particlebombardment)转化技术成功地将豇豆胰蛋白酶抑制剂(cowpeatrypsininhibitor,CpTI)基因分别导入三个水稻粳稻栽培品种 (OryzasativaL .,japonica)中花 8、中花 10和中作 32 1的幼胚和胚性愈伤组织中 ,并由此获得了可育的再生植株 ,抗性植株的筛选频率为 6.1%～ 12 .2 4 %。经PCR、Southernblot分子检测和ELISA检测等证实所获得的潮霉素抗性植株为转基因植株。抗虫性分析结果表明 ,部分转基因水稻植株对玉米螟虫 (Ostrinianubilalis)A cowpea trypsin inhibitor (CpTI) gene was successfully transferred into immature embryos and embryogenic calli of three varieties of rice, Zhonghua 8 , Zhonghua 10 and Zhongzuo 321 via the particle bombardment. Fertile transgenic rice plants were obtained, and confirmed by assay of PCR, Southern blot and ELISA. Selective frequencies of HygB resistance plants were among 6.1%～12.4%. Resistant insect bioassay indicated that some of transgenic rice plants shown good resistance to corn borer (Ostrinia nubilalis).

关 键 词：胰蛋白酶抑制剂 抗虫基因 转基因水稻 生物检测 基因枪法 

分 类 号：Q943.2[生物学—植物学]