流动注射——分光光度法测定药剂中维生素C的方法研究  

Determination of Vitamin C in Medicament with Spectrophotometry of FIA

作　　者：王凤兰[1] 金山[1] 孟玉玲 赵珍义[3] 

机构地区：[1]沈阳医学院预防医学系卫生化学教研室,沈阳110031 [2]沈阳医学院卫生所 [3]辽宁大学科学与工程学院,沈阳110026

出　　处：《沈阳医学院学报》2001年第1期6-8,共3页Journal of Shenyang Medical College

摘　　要：目的　建立测定药剂中维生素C含量的分析方法。方法　采用流动注射技术与分光光度法相结合。用 0 0 5%的Fe(Ⅲ ) - 1 ,1 0 -二氮杂菲为显色剂 ,在入max为 50 4nm处测定吸光度值。结果　标准曲线在 0 0 0 6 0 0 μg/ml范围内线性良好 ,平均回收率 98 4% ,RSD为 0 2 6% 0 98% ,检出限 0 0 2 μg/ml。结论　此方法测定药剂中维生素C含量 ,结果准确。Objective To establish an analysis method of vitamin C contents with spectrophotometry of flow injection analysis (FIA) in medicament. Methods FIA technique was used with spectrophotometry together, with 0 05% 1 10-Nitro-0-phenanthroline iron(Ⅲ) as authrone color reagent. Determine numerical value of absorbance at λmax: 504 nm. Results The standard curve of macrostemonoside G was linear in the concentration range of 0 00~6 00 μg/ml. The average recovery was 98 4%. RSD was 0 26%~0 98%. Conclusion The method is dependable, accurate and easy to operate.

关 键 词：流动注射分析 分光光度法 维生素C 

分 类 号：R977.23[医药卫生—药品] R927.2[医药卫生—药学]