青蒿素衍生物抗卡氏肺孢子虫体外作用的研究  

In vitro study of the anti-pneumocystis carinii effect of arteminsin derivatives

作　　者：倪小毅[1] 陈雅棠[1] 

机构地区：[1]重庆医科大学第一临床学院传染病寄生虫病研究所,400016

出　　处：《中华结核和呼吸杂志》2001年第3期164-167,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：国家自然科学基金资助项目!(396 70 6 5 8)

摘　　要：目的　研究不同浓度青蒿素衍生物双氢青蒿素、青蒿琥酯对体外培养卡氏肺孢子虫的作用。方法　接种卡氏肺孢子虫 (Pc)于人肝癌细胞系 (HepG 2 )。 4h后加入试验药物和对照试剂。双氢青蒿素药物浓度分别为 :10 0、5 0、10、5、0 5 μmol/L ;青蒿琥酯药物浓度分别为 :10 0、5 0、10、5 μmol/L ;对照药喷他脒 :15 μmol/L。以后每隔 1d取培养液观察 ,分别作六亚甲基四胺银 (GMS)和Diff Quik (DQ)染色计数Pc包囊和滋养体数目。结果　Pc滋养体和包囊在体外培养细胞上的生长高峰出现于第 5d ,双氢青蒿素浓度为 5 0 μmol/L和 10 0 μmol/L时对Pc滋养体的抑制率分别为 88 0 %与90 1% ,与喷他脒 15 μmol/L的抑制率 90 0 %相当 ;而青蒿琥酯浓度为 10 0 μmol/L时方可达到Pc滋养体抑制率 83 4%。Pc包囊抑制率 ,双氢青蒿素和青蒿琥酯浓度为 10 0 μmol/L时分别为 6 7 5 %和6 7 3% ,与喷他脒的抑制率 75 0 %之间无统计学差异。结论　双氢青蒿素和青蒿琥酯在一定浓度时对Pc的抑制作用与喷他脒相当 ;而双氢青蒿素的抑制作用略高于青蒿琥酯。Objective In vitro study was carried out to assess the effect of dihydroarteminsinin(DHA) and sodium artesunate(ATS) on pneumocystis carinii(Pc). Methods The drugs were added into HepG 2 cell cultures containing Pc cysts The concentrations of dihydroarteminsinin (DHA) were 100, 50, 10, 5, 0 5 μmol/L respectively The concentrations of sodium artesunate (ATS) were 100, 50, 10, 5 μmol/L respectively and pentamidine (PTD) was 15 μmol/L as a control The supernatants were withdrawn every other day and evaluated for Pc trophozoite and cyst counts by GMS and DQ stain. Results The peak of Pc multiplication was observed on 5th day The inhibition rates of Pc trophozoites by DHA 50 μmol/L, 100 μmol/L and ATS 100 μmol/L were quite similar to that of pentamidine (PTD) 15 μmol/L. Conclusion DHA and ATS can inhibit the growth of Pc as same as PTD, and DHA was slightly better than ATS

关 键 词：卡氏肺孢子虫 双氢青蒿素 青蒿琥酯 喷他脒 实验研究 

分 类 号：R965[医药卫生—药理学]