补肾益智方对老年性痴呆模型大鼠海马结构生长抑素神经元的影响  

Effects of Bushen Yizhi Decoction on Somatostatin-like Neurons in Hippocampal Gyrus of Rat Models with Alzheimer's Disease

作　　者：张魁华[1] 赖世隆[1] 胡镜清[1] 王奇[1] 梁伟雄[1] 程淑意[1] 

机构地区：[1]广州中医药大学DME中心

出　　处：《广州中医药大学学报》2001年第1期25-29,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家"九五"攻关课题!(编号 :96 - 96 - 0 90 2 );国家医药技术博士创新基金课题 !(编号 :98-B - 13)

摘　　要：【目的】研究自拟的补肾益智方对大鼠老年性痴呆 (阿尔茨海默病 ,Alzheimerdisease ,AD)模型海马内生长抑素 (somatostatin ,SS)神经元的影响。【方法】用 15月龄老年Wistar大鼠以D -半乳糖腹腔注射 4周加上鹅膏蕈氨酸 (ibotenicacid ,IBO)脑内Meynert核注射制作AD模型 ,然后随机分成AD模型组、双益平治疗对照组、补肾益智方高剂量治疗组、补肾益智方低剂量治疗组 ,另设正常老年对照组和正常青年对照组。动物经治疗处理后 ,运用免疫组织化学和寡核苷酸探针原位杂交方法检测大鼠海马CA 1、CA 3和齿状回生长抑素免疫阳性和mRNA表达阳性神经元数目。【结果】补肾益智方高、低剂量组大鼠海马CA1、CA3和齿状回生长抑素阳性神经元数目及其积分光密度较AD模型组有增加趋势 ,有些组间比较有显著性差异。【结论】补肾益智方对D -半乳糖腹腔注射 4周加上鹅膏蕈氨酸 (IBO)脑内Meynert核注射制作的大鼠AD模型海马内生长抑素 (SS)Objective To observe the effects of Bushen Yizhi Decociton (BYD) on somatostatin-like (SS-like)neurons in hippocampal gyrus of rat models with Alzheimer's disease(AD). Methods Fifteen-month_old AD rat models were established by intraperitoneral injection of D-galactose for 4 weeks combined with ibotenic acid injection into bilateral nucleus basalis of Meynert. AD model rats were randomly allocated to AD model group(Group C), Hup-A treatment group(Group D) and BYD treatment groups (Group E and Group F,treated with high dosage and low dosage respectively), and 10 normal aged rats (Group B)and 10 normal youth rats (Group A)served as the normal control groups. The methods of immunohistochemistry and dig_labeling c-DNA probe in situ hibridization were used to detect the number of SS-like immunoreactive positive and SS mRNA expressed positive neurons in hippocampal CA1 and CA3 fields and the dentate gyrus. Results The number and optic density of SS-like immunoreactive positive and SS mRNA expressed positive neurons were higher in BYD treatment groups than those in Group C (P< 0.05 ?or? 0.01 ).The differences of the above indexes among some groups were significant (P< 0.05 ?or? 0.01 ). Conclusion BYD possesses a certain protective effect on SS-like neurons in hippocampal gyrus of rat models with AD.

关 键 词：补肾益智方 药理学 老年性痴呆 中医药疗法 海马结构 实验研究 生长抑素 神经元 

分 类 号：R277.791.6[医药卫生—中医学] R285.5