中国芦荟离体培养和快速繁殖  

In-Vitro Culture and Rapid Propagation of Aloe vera L.Var.chinensis(Haw.)Berger

作　　者：贺红[1] 刘春来[2] 肖省娥[1] 徐鸿华[1] 方斌斌[2] 梁丽仪[2] 

机构地区：[1]广州中医药大学中药资源研究室,广州510405 [2]广州中医药大学中药学院,97级本科生广州510405

出　　处：《广州中医药大学学报》2001年第1期71-73,共3页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】探讨采用植物组织培养技术进行中国芦荟的快速繁殖 ,为工厂化育苗提供技术依据。【方法】以中国芦荟地下茎发生的幼芽为外植体 ,诱导丛生芽的产生。【结果】基本培养基选择MT (MurashigeandTucker)较好 ;培养基中附加 6_苄基氨基嘌呤 ( 6_Benzylaminopurine ,BA)能提高成芽率 ,并能增加外植体出芽的数量 ;BA浓度为 2mg·L_1时 ,有利于芽的诱导与生长 ,出芽率达 10 0 % ;根的诱导 ,以MT +0 .5mg·L_1萘乙酸 (Naphthaleneacid ,NAA)培养基较好 ,生根率为 10 0 %。【结论】通过培养芦荟地下茎发生的幼芽 ,既可以迅速建立快速繁殖体系 。Objective To find out technological evidence for the rapid propagation of Aloe vera L.var.chinensis (Haw.)Berber. Methods The technique of plant tissue culture was used to study the rapid propagation of Aloe vera L.Var.Chinesis(Haw.)Berger.The shoot growing from the underground stem of Aloe vera L.Var.chinensis(Haw.)Berger was taken as the explant to induce fascicular bud. Results The optimal culture medium was MT.MT culture medium added with 6-benzylaminopurine(BA) was effective to induce the shoot formation and increase the direct shooting rate.The optimal BA concentration for the induction and formation of shoot was 2 mg·L -1 ,the shooting rate being 100%.As for the induction of rooting,MT culture medium adding with 0.5 mg·L -1 naphthalene acid was optimal,the rooting rate being 100%. Conclusion The culture of Aloe shoot growing from the underground stem,proper culture medium and BA concentration are important for the rapid propagation of Aloe.

关 键 词：芦荟 生长 发育 离体培养 快速繁殖 

分 类 号：S567.239[农业科学—中草药栽培]