α_1-酸性糖蛋白柱拆分沙美特罗对映体  

Enantiomeric Separation of Salmeterol by HPLC Using α_1-Acid Glycoprotein Chiral Stationary Phase

作　　者：杨永健[1] 侯美琴[1] 

机构地区：[1]上海市药品检验所,上海200233

出　　处：《药物分析杂志》2001年第2期97-100,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：上海市卫生局资助课题;上海市药检所资助课题!编号 98419

摘　　要：目的 :建立沙美特罗对映体拆分方法。方法 :以α1 -酸性糖蛋白 (α1 -AGP)为固定相 ,考察了流动相pH、有机改性剂及流速对分离的影响。结果 :以 10mmol·L- 1 醋酸钠 (pH 4 0 ) -异丙醇 (98∶2 )为流动相 ,流速为 0 9mL·min- 1 ,在α1 -AGP柱上拆分了沙美特罗。结论 :流动相pH和有机改性剂的量是优化分离带正电荷对映体的分离的重要因素。同时可以在一定的流速范围内通过调节流速来提高柱效、改善分离。Objective:To develop an enantiomeric separation method of salmeterol by HPLC using α 1-acid glycoprotein (α 1-AGP)chiral stationary phase.Method:Influences of the pH value of the mobile phase,organic modifiers,and flow rate on the enantiometric separation of salmeterol using α 1-AGP chiral stationary phase were investigated.Results:Salmeterol enantiomers were separated on an α 1-AGP chiral stationary phase column with a mobile phase consisted of 10 mmol·L -1 NaAc(pH 40)-isopropanol(98∶2)at a flow rate of 09 mL·min -1.Conclusion:The pH value of the mobile phase and the organic modifier concentration were very important parameters when optimizing the enantiomeric separation of the cationic racemates.Higher column efficiency was obtained with lower flow rates.

关 键 词：沙美特罗 对映体 手性拆分 Α1-酸性糖蛋白 

分 类 号：R975[医药卫生—药品]