不同类型银杏叶中银杏内酯的含量测定  

Determination of Ginkgolides in Various Ginkgo Leaves

作　　者：管玉民 尤慧莲 王健 林晓 

机构地区：[1]山东省药品检验所,济南250012

出　　处：《药物分析杂志》2001年第2期107-109,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：山东省中医药科技发展规划项目!No 972 463 4

摘　　要：目的 :建立HPLC法测定各种类型银杏叶中银杏内酯的含量。方法 :色谱柱为SUNIERKromasilC1 8(5 μm ,4 6mm×2 5 0mm) ,以 33%甲醇为流动相 ,流速 :1mL·min- 1 ,示差检测器 (RI) ,以银杏内酯A、B、C及白果内酯 4种对照品为对照。结果 :测定了各种类型的银杏叶内酯的含量。结论 :找出了银杏叶的最佳采集时间。Objective:To determine ginkgolides in various Ginkgo leaves by HPLC.Method:The analysis was performed on SUNIER Kromasil C 18 column(5 μm,46 mm×250 mm) with 33% methanol as mobile phase,at a flow rate of 1 mL·min -1.The differential refractometer was used as detector and ginkgolide A\,B\,C and bilobalide were used as comparison.Result:The content of ginkgolides in various Ginkgo leaves have been determined.Conclusion:The best time of picking Ginkgo leaves was defined.

关 键 词：银杏叶 银杏内酯 白果内酯 高效液相色谱法 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]