用基因枪法将AGP基因导入水稻的初步研究(英文)  

A Preliminary Study on the Transformation of ADP Glucose Pyrophosphorylase Gene Using Particle Bombardment

作　　者：李援亚[1] 王力[2] 张云孙[1] 

机构地区：[1]云南大学生物系,云南昆明650091 [2]成都军区昆明总医院病理科,云南昆明650031

出　　处：《云南大学学报（自然科学版）》2001年第2期146-148,152,共4页Journal of Yunnan University(Natural Sciences Edition)

基　　金：ProjectSupportedbytheNaturalBasicResearchFoundationofYunnanProvince( 96C0 0 3Z) .

摘　　要：以水稻成熟种子的愈伤组织为受体 ,采用基因枪法将AGP基因导入水稻细胞 ,通过组织培养和抗性筛选 ,得到了转基因植株 .转基因植株总DNA的PCR分析初步表明 ,目的基因已整合到水稻的基因组中 .The genes of AGP (ADP glucose pyrophosphorylase)were introduced into calli induced from embryos of mature rice seeds using particle bombardment.Transgenic plants were obtained by tissue culture and resistance selection.PCR analysis of the total DNA extracted from transgenic plants has shown that the target gene had been integrated into the rice genome.

关 键 词：水稻 愈伤组织 AGP基因 植株再生 基因枪法 转基因植株 组织培养 PCR分析 

分 类 号：S511.035.3[农业科学—作物学]