几种激素和IL-1β对人脑星形胶质细胞IL-6、TNF-α分泌的影响  

Effect of Some Hormones and IL-1β on the Production of IL-6 and TNF-α in Astrocytes from Human Fetal Brain in Vitro

作　　者：李兰英[1] 孙云[1] 庞智玲[1] 

机构地区：[1]天津医科大学内分泌研究所,天津300070

出　　处：《中国神经免疫学和神经病学杂志》2001年第2期112-115,共4页Chinese Journal of Neuroimmunology and Neurology

基　　金：天津市科委自然科学基金资助项目! (963 10 8411)

摘　　要：目的　观察 T3等因素对体外培养人胎大脑星形胶质细胞分泌 IL-6、TNF-α的调节作用。方法　纯化培养人胎大脑星形胶质细胞 ,应用酶联免疫分析 (ELISA)方法检测培养上清液中 IL-6、TNF-α的水平。结果　 (1 )星形胶质细胞 (AC)在体外培养条件下可自发分泌 IL-6,而 TNF-α则几乎检测不到。(2 ) LPS(0 .1μg/ m L)即可诱导AC产生 IL-6和 TNF-α。 (3 ) IL-1 β是 IL-6分泌的主要诱导剂 ,但不诱导 TNF-α分泌。 (4 )氢化可的松可明显抑制 AC分泌 IL-6、TNF-α。(5) T3在 72 h可刺激 IL-6的分泌。 (6)胰岛素对 IL-6的分泌没有明显的调节作用。结论　 AC可通过分泌细胞因子参与炎症反应等病理过程并维持中枢神经系统的正常发育、内环境的稳定 ,且受多种因素的调节。在中枢神经系统中 T3、胰岛素主要参与调节发育和代谢 ,可能不直接参与炎症和免疫机制调节。Objective Studing the effect of some hormons and IL-6 on the production of IL-6 and TNF-α in astrocytes from human fetal brain. Methods The purified cultures of astrocytes were prepared from second-trimester human fetal brain. The levels of IL-6 and TNF-α were detected by ELISA before and after stimulation with IL- 1β,LPS , insulin, triiodothyronine(T 3)and hydrocortisone. Results (1)Before stimulated the level of IL-6 in medium was (73.60±9.14)pg/mL at 24 h and(68.46±22.60)pg/mL at 72 h respectively and few TNF-α were detected. (2)LPS(0.1 μg/mL) could induce the production of IL- 6 and TNF-α in astrocytes (P <0.001).(3)IL-1β was a strong stimulus for production of IL-6 in a dose-dependent fashion and the level of IL-6 induced by IL-1β(100 U/mL) is 30 fold more than the base level . IL-1β could not stimulate TNF-α secretion . (4) T 3 (10 -8 mol/L)could increase the level of IL-6 at 72 h(P<0.05) . (5) Hydrocortisone suppress the production of IL-6 and TNF-α before and after stimulation with IL-1β and LPS . (6)Insulin failed to induce IL-6 and TNF-α in astrocytes. Conclusions It is suggested that cytokines produced by astrocytes play the role in inflammation and maintain the normal homeostatic environment of CNS. T 3 and insulin basically regulate the development and metabolism of astrocyte but not directly affect immune respones in CNS.

关 键 词：星形胶质细胞 细胞因子 三碘甲状腺氨酸 

分 类 号：R335[医药卫生—人体生理学] R58[医药卫生—基础医学]