实验性NIDDM模型胰岛素抵抗的评估  

Insulin Resist Analysis in Non Insulin-Dependent Diabetic Rat Model

作　　者：刘欣秋[1] LEI Ming 张桂芳[1] 雷鸣[3] 赵安飞 

机构地区：[1]黑龙江省佳木斯大学基础医学院生理教研室,佳木斯154002 [2]Department of Physiology,Basic Medical College of Jiamusi University, [3]佳木斯大学第二附属医院 [4]农垦总局医院佳木斯

出　　处：《中国误诊学杂志》2001年第4期492-494,共3页Chinese Journal of Misdiagnostics

摘　　要：目的　用糖耐量曲线下面积 (SBG)、胰岛素释放曲线下面积 (SIns)、胰岛素敏感指数 ,这些临床糖尿病常用的指标对非胰岛素依赖型糖尿病 (non insulin- dependentdiabetes mellitus,NIDDM)模型胰岛素抵抗评估。方法　大鼠尾静脉注射小剂量链尿佐菌素 ,使大鼠糖耐量异常 ,然后加喂高热量食物 ,引起动物肥胖 ,饲养 8wk,可形成类似 NIDDM的肥胖大鼠模型 ,并设置对照组 ,同时测定了大鼠糖耐量试验过程中血清葡萄糖 (BG)、胰岛素 (Ins) ,并计算了 SBG、SIns、胰岛素敏感指数。结果　 1模型组糖耐量试验 ,血清 BG糖耐量下降 ,Ins在糖负荷后 2 h明显升高 (P<0 .0 1) ;2 Ins敏感指数较对照组明显降低 (P<0 .0 1) ;3SBG明显上升 (P<0 .0 1) ;SIns无明显变化 ,SIns/ SBG下降 (P<0 .0 1)。结论　SBG、SIns、胰岛素敏感指数可较好反映 Ins作用 ,B细胞储备功能及 Ins敏感性 ,可广泛用于实验性糖尿病基础研究和作为临床正确评价 IR的指标。Objective To analyse insulin resist (IR) of non insulin dependent diabetic rat model with insulin sensitive index,the area of and glucose under curve (SBG),the area of insulin under curve (SIns),SIns/SBG which were used in diabetic patient.Methods Rats were injected with small dose streptozocoi (iv).Two weeks after the injection,the rats developed impaired glucose tolerance.Then,they were fed with high energy diet for eight weeks to form non insulin dependent diabetes mellitus (NIDDM).Concentrations of blood glucose and serum insulin in oral glucose tolerance were detected.SBG,Sins which is used describing IR and function of pancreas β cell are also calculated.Results ①The glucose tolerance was impaired,hyper insulin was found in the oral glucose tolerance test(P<0.01).②Insulin sensitive index decreased compared with controls (P<0.01).③SBG was significantly higher than that of control (P<0.01),but no significant difference was found with SIns between two groups.SIns/SBG decreased compared with controls(P<0.01).Conclusions Insulin sensitive index、SBG、SIns/SBG may reflect affect of insulin、function of pancreas β cell and insulin sensityvity,which were widely applied to basic research of experimental diabetes,or as evaluated index of IR.

关 键 词：非胰岛素依赖性糖尿病 血糖 胰岛素敏感指数 糖耐量曲线下面积 胰岛素释放曲线下面积 

分 类 号：R587.1[医药卫生—内分泌]