铝盐致大鼠学习记忆功能障碍的β-APP、SS作用机制研究  

The roles of β APP and SS in the deficits of learning and memory function in rats due to subcutaneous injection of aluminum salt

作　　者：王怀明[1] 杨望清 郭洪志[3] 刘爱芬[3] 迟兆富[3] 

机构地区：[1]山东潍坊89医院,261021 [2]山东济南106医院 [3]山东医科大学附属医院

出　　处：《临床神经病学杂志》2001年第1期24-26,共3页Journal of Clinical Neurology

摘　　要：目的　观察 β淀粉样前体蛋白 (APP)、生长抑素 (SS)在铝盐致大鼠学习记忆功能障碍模型脑内海马区表达的改变 ,并探讨其作用机制。方法　采用免疫组织化学方法检测 β- APP、SS在大鼠脑内海马区的表达 ,HE染色观察实验组与对照组海马区锥体细胞层神经元形态结构。结果　实验组大鼠学习记忆功能较对照组明显降低 (P<0 .0 1) ;实验组海马区 β- APP表达较对照组明显增加 (P<0 .0 1) ;SS免疫反应性神经元数目却显著减少 (P<0 .0 1) ;β- APP阳性度与 SS阳性度之间存在等级负相关 (P<0 .0 5 ) ,实验组与对照组海马区神经元形态结构均基本正常 ,数目无明显差异。结论　铝盐致大鼠学习记忆障碍的病理机制可能与脑内β- APP。Objective To observe the expression of β APP and SS in the hippocampus of the rat mode ls with the deficits of learning and memory function, and investigate its mechanism.Methods Using immunohistochemistry (IHC), we detected the expressions of β amyloid precursorprotein(β APP) and somatostatin(SS) in the hippocampus of the rat brains,using HE staining method, we observed the shapes and structures of pyramidal cell layer neurons in hippocampus of the rats in the experimental group and the controls.Results The learning and memory functions were lower significantly in the experimental group than in the controls ( P <0.01);The expression of β APP in hippocampus of experimental group was significantly higher than the controls ( P <0.01);but the number of SS immunoreactive positive neurons decreased sigificantly ( P <0.01); There was negative relation between the positive degrees of β APP and SS in the experimental group ( P <0.05).The structures of neurons in hippocampus of the experimental group and the controls were fundamental normal,there was no significant difference between the numbers of their neurons.Conclusion The pathogenesis of aluminum slat resulted in the deficits of learning and memory functions in rats,it might be related to the changes of the expressions of β APP and SS in brain.

关 键 词：铝 Β-淀粉样前体蛋白 生长抑素 学习记忆 

分 类 号：R99[医药卫生—毒理学]