套式PCR扩增特定SSU rRNA基因片段检测四川疟原虫感染的研究  

DETECTION OF HUMAN MALARIA PARASITES BASED ON AMPLIFYING SPECIFIC SSU rRNA GENE FRAGMENT BY NESTED PCR IN SICHUAN, CHINA

作　　者：姜素华[1] 刘佩娜[1] 朱声华[1] 刘庆 陈建平[1] 

机构地区：[1]华西医科大学寄生虫学教研室,四川成都610041 [2]Infectious Disease Section Department of Internal Medicine

出　　处：《实用寄生虫病杂志》2001年第1期4-7,共4页Journal of Practical Parasitic Diseases

基　　金：日本丰田基金! (96B3 -0 11)

摘　　要：目的用套式 PCR系统诊断、鉴别人体疟原虫感染。方法以疟原虫小亚单位核糖体核糖核酸 (SSUr RNA)基因为靶基因 ,选用 1对疟原虫属特异性引物和 4对种特异性引物 ,建立套式 PCR扩增系统并用于四川省疟疾病人血样的检测。结果从间日疟原虫、恶性疟原虫和三日疟原虫感染血样中分别扩增出 1 0 4 bp、1 0 2 bp和 1 1 5bp预期大小的特定扩增带。61份血样检测结果与镜检的间日疟阳性符合率为 1 0 0 %。并查出镜检漏诊的 2例 P.v.和 P.m.的混合感染及 1例 P.v.、P.f.和 P.m.的混合感染病例。结论本系统特异、灵敏、稳定、简便 ,可在诊断疟疾的同时判定混合感染 ,故对疟疾的诊断、大规模流行病学研究及疫情监控有实际应用价值。Aim To detect and identify human malaria parasites by nested PCR. Methods A pair of genus primer and four pairs of species-specific primers are chosen to establish nested PCR system and the targets specific sequence in the SSU rRNA gene are used for detecting blood samples obtained from malaria patients in Sichuan. Results 104bp,102bp and 115bp DNA expected fragments were amplified from the blood samples of 61 patients with P.vivax ,P.falciparum and P.malariae infections respectively. The positive concordance rate between nested PCR and microscopic examination was 100% in detecting P.vivax. In addition , 3 cases of mixed Plasmodium infections were identified by nested PCR. Conclusion The system is specific, sensitive and stable in detecting mixed malatia infections. It would provide a practical tool in diagnosis and epidemiological survey of malaria.

关 键 词：间日疟原虫 恶性疟原虫 疟疾 卵形疟原虫 套式聚合酶链反应 小亚单位核糖 

分 类 号：R531.3[医药卫生—内科学] R382.31[医药卫生—临床医学]