流式细胞术检测红细胞相关抗体及临床应用  

Detection of erythrocyte associated immunoglobulin by flow cytometry

作　　者：钟明华[1] 廖军鲜[1] 龙红[1] 许勇钢[2] 杨晓红[2] 

机构地区：[1]中日友好医院血液科,北京100029 [2]中国中医研究院西苑医院血液科,北京100091

出　　处：《中日友好医院学报》2001年第1期3-6,共4页Journal of China-Japan Friendship Hospital

摘　　要：目的 :建立流式细胞术 (FCM )检测红细胞相关抗体 (EAIg)的方法 ,以用于EAIg的检测、定量和分型。方法 :利用流式细胞仪和抗人IgG、IgA、IgM单克隆抗体检测EAIg及其亚型。 结果 :免疫性溶血性贫血 (IHA)患者IgG(+)红细胞百分率显著高于正常组 (P <0 0 5 ) ,IgA、IgM阳性率也均达 2 0 7%。本法结合单特异性抗C3 血清可对IHA行进一步免疫学分型。结论 :FCM对EAIg进行定量测定及分型 ,方法灵敏、结果客观。Objective:To establish a flow cytometry (FCM) method for detection and immunophenotyping of erythrocyte associated immunoglobulin(EAIg).Methods:Flow cytometre and monoclonal antibodies were used to detect and analyze EAIg and their subsets.Results:The percentage of IgG positive red blood cell in immune patients with hemolytic anemia(IHA)was significantly higher than that of control group( P <0 05).IgA and IgM positive rates were both 20 7%.In IHA patients,immunophenotyping were performed by FCM.Conclusion:This method is not only sensitive,rapid and proper,but also show us the subsets of autoantibodies.It is helpful in the diagnosis of IHA.

关 键 词：溶血性贫血 自身免疫性 流式细胞术 检测 

分 类 号：R446.11[医药卫生—诊断学]