截短型乙型肝炎病毒表面抗原中蛋白反式激活作用的初步研究  

Preliminary study of transcriptional transactivator function generated by C-terminal truncated middle surface protein of the hepatitis B virus

作　　者：刘妍[1] 成军[1] 董菁[1] 李克 夏小兵[1] 王刚[1] 杨继珍[1] 王琳[1] 

机构地区：[1]北京解放军第三0二医院传染病研究所基因治疗研究中心,100039

出　　处：《肝脏》2001年第1期8-10,共3页Chinese Hepatology

基　　金：军队回国留学人员启动资金资助! (98H0 38)

摘　　要：目的　探讨乙型肝炎病毒 (HBV )表面抗原中蛋白羧基末端缺失突变体 (MHBst)的反式激活功能。方法　用聚合酶链反应 (PCR)扩增截短的HBV表面抗原中蛋白 (MHBst167)基因 ,克隆至真核表达载体 pcDNA3 .1(-)中 ,转染COS 7细胞 ,酶联免疫吸附法 (ELISA)检测细胞MHBst167蛋白的瞬时表达 ;与报告质粒 pSV lacZ共转染COS 7细胞 ,检测 β 半乳糖苷酶的活性。 结果　构建成含有约 5 0 0bp基因的质粒 pcDNA3 .1(-) Mt ,在COS 7细胞表达出相应蛋白 ,对pSV lacZ的表达具有反式激活作用。 结论　构建的表达载体能在哺乳动物细胞中表达 ,并具有反式激活功能。Objective To investigate the transactivator function of the C terminal truncated middle surface protein of the hepatitis B virus. Methods Polymerase chain reaction was employed to amplify the coding sequence of MHBs t167 from plasmid pCP10, and the product was cloned into pcDNA 3.1(-), then transfected the COS 7 cell line. MHBs t167 was detected by ELISA method. Cotransfected COS 7 cell with reporter plasmid pSV lacZ and activator plasmid pcDNA 3.1(-) Mt, the activity of β galactosidase was detected.Results MHBs t167 coding sequence was cloned and sequenced. After being transfected into COS 7 cell line, MHBs t167 was expressed and detected of which the transactivator function was proven by cotransfection test.Conclusion pcDNA 3.1(-) Mt can be expressed in mammalian cell line, and has transcriptional transactivator function.

关 键 词：乙型肝炎病毒 截短 反式激活 表面抗原 MHBs^t 

分 类 号：R373[医药卫生—病原生物学]