钌红对严重烧伤早期心肌能量代谢的影响  

Effects of ruthenium red on myocardial energy metabolism during the early stage after severe burns

作　　者：梁晚益[1] 杨宗城[1] 黄跃生[1] 

机构地区：[1]第三军医大学西南医院烧伤研究所,重庆400038

出　　处：《中华实验外科杂志》2001年第2期163-164,共2页Chinese Journal of Experimental Surgery

基　　金：国家重点基础研究发展规划项目 (G1 9990 542 0 2 )

摘　　要：目的　探讨钌红对严重烧伤早期心肌能量代谢的影响。方法　Wistar大鼠 2 4只 ,随机分为正常对照组、烧伤组和烧伤钌红治疗组。烧伤组、烧伤钌红治疗组大鼠造成 3 0 %总体表面积Ⅲ度烧伤 ,伤后 3 0min经腹腔补液 ,烧伤治疗组同时于颈外静脉推注钌红 (2mg/kg体重 ) ,3h后再推注 1次。烧伤组和烧伤治疗组动物于伤后 6h活杀。测定心肌线粒体呼吸功能、Ca2 + 浓度([Ca2 + ] m)及心肌组织ATP、ADP、AMP和乳酸含量。结果　钌红治疗组 [Ca2 + ] m 较烧伤组显著降低 ,线粒体呼吸控制率 (RCR)、Ⅲ态呼吸速率 (ST3 )明显升高 ,Ⅳ态呼吸速率 (ST4 )降低 ;钌红治疗组ATP含量较烧伤组升高 10 0 .4% ,同时ADP、AMP含量明显低于烧伤组 ,且钌红治疗组乳酸含量较烧伤组降低 5 3 .5 %。Objective To investigate the effects of ruthenium red on myocardial energy metabolism during the early stage after severe burns. Methods Wistar rats were randomized into normal control group, burns group and ruthenium red treated group (RR) ( n =8 in each group). The rats in the burns group and RR group were scalded to 30% total body surface area degree Ⅲ, and 30 min later, resuscitated with Ringer's solution. Meanwhile, ruthenium red was injected to the rats of RR in a dose of 2 mg/kg, and once again 3 h later. After rats were sacrificed at the 6th h postscalding, contents of myocardial ATP, ADP, AMP and lactic acid, mitochondrial respiratory function and Ca 2+ concentration([Ca 2+ ] m) were determined. Results Myocardial ATP content in the RR group was increased by 100.4% and lactic acid decreased by 53.5% as compared to the burns group. Meanwhile, mitochondrial respiratory control rate (RCR) and ST 3 were higher than those of burns group. However, [Ca 2+ ] m, ST 4, ADP and AMP in the RR group were significantly lower than those in the burns group. Conclusion Ruthenium red improved myocardial energy metabolic conditions and mitochondrial function during the early stage after severe burns.

关 键 词：烧伤 钌红 三磷酸腺苷 线粒体 心肌能量代谢 

分 类 号：R644[医药卫生—外科学] R654.2[医药卫生—临床医学]