[Ca^(2+)]i在低剂量辐射诱导脾细胞免疫适应性反应中的作用  

THE ROLE OF [Ca^(2+) ]i MOBILIZATION OF SPLENOCYTES IN IMMUNE ADAPTIVE RESPONSE INDUCED BY LOW DOSE RADIATION

作　　者：傅海青[1] 鞠桂芝[1] 

机构地区：[1]白求恩医科大学卫生部放射生物重点实验室,长春130021

出　　处：《辐射防护》2001年第2期115-118,共4页Radiation Protection

基　　金：国家自然科学基金!资助课题 (39170 2 75 )

摘　　要：为探讨胞浆内游离钙离子浓度 ( [Ca2 +]i)在低剂量辐射诱导脾细胞免疫适应性反应中的作用 ,本文采用 Fura- 2 /AM双波长荧光测定法观察了不同剂量 X射线全身照射后小鼠脾细胞内 [Ca2 +]i的变化及低剂量辐射诱导的适应性反应。结果表明 ,小鼠接受 1.0～ 6.0 Gy X射线全身照射后 2 4 h,脾细胞内[Ca2 +]i呈剂量依赖性下降 ( p <0 .0 5～ p <0 .0 1) ,而小鼠接受 0 .0 75～ 0 .2 Gy较低剂量 X射线全身照射后 2 4 h,脾细胞内 [Ca2 +]i却明显高于假照射组 ( p<0 .0 1)。同时证实 ,预先给予 0 .0 75Gy低剂量 X射线全身照射可减轻其后 2 .0 Gy X射线全身照射对脾细胞内 [Ca2 +]i的抑制作用。提示低剂量辐射诱导脾细胞内 [Ca2 +]i的升高可能在低剂量辐射诱导脾细胞免疫适应性反应中起重要作用。To elucidate the role of [Ca 2+ ]i mobilization of splenocytes in immune adaptive response induced by low dose radiation , dose effects and adaptive response of intracellular [Ca 2+ ]i concentration of splenocytes in Kunming mice following whole body irradiation WBI with X rays were studied by using fura 2 as a fluorescent indicator. Results indicated that WBI with 1.0~6.0 Gy X rays could decrease the [Ca 2+ ]i concentration of splenocytes in a dose dependent manner 24 h after irradiation in mice. However, [Ca 2+ ]i concentration of splenocytes in mice irradiated with 0.075~0.2 Gy X ray WBI was significantly higher than that irradiated with 0 Gy. Pre irradiation with 0.075 Gy WBI could markedly reduce the inhibition of [Ca 2+ ]i in splenocytes by 2.0 Gy WBI. In conclusion, [Ca 2+ ]i mobilization in T lymphocyte might play an important role in immune adaptive response induced by low dose radiation.

关 键 词：电离辐射 脾细胞 免疫适应性反应 游离钙离子浓度 免疫增强 

分 类 号：R392.12[医药卫生—免疫学]