体细胞HPRT基因突变检测用于辐射剂量估算的可行性研究  

FEASIBILITY STUDY OF RADIATION DOSE ESTIMATION USING SOMATIC HPRT GENE MUTATION

作　　者：段志凯[1] 陈英[1] 张淑贤[1] 徐洪兰[1] 王明明[1] 

机构地区：[1]中国辐射防护研究院,太原030006

出　　处：《辐射防护》2001年第2期119-122,共4页Radiation Protection

摘　　要：用多核细胞法研究了 60 Coγ射线 0～ 4 .0 Gy照射诱发的体细胞 HPRT基因突变 ,探讨了其在辐射剂量估算中的适用范围和存在的问题。结果表明 ,HPRT基因突变频率 Y随照射剂量 D ( Gy)的增加而增加 ,在 0～ 4 .0 Gy剂量范围内可拟合为 Y=0 .4 0 2 2 +0 .2 710 D - 0 .0 4 60 D2 ,在 0～ 1.0 Gy低剂量范围内 ,更适于拟合直线模型 Y =0 .382 7+0 .2 94 8D;HPRT基因突变频率与染色体畸变、微核具有良好的相关性。说明 HPRT基因突变可以用于低 LET辐射剂量估算 ,尤其适用于低剂量照射引起的单基因突变检测和辐射危害评价。Somatic HPRT gene mutations induced by exposure of 0~4 Gy 60 Co γ ray have been studied with the multinuclei cell method. The scopes of application and problems to be encountered in the radiation bio dose estimation using somatic HPRT gene mutations were approached. It was shown that the frequency of HPRT gene mutation was fitted in with a linear quadratic model of Y=0.4022+0.2710D-0.0460D 2 in the 0～4.0 Gy range and more fitted to a linear model of Y=0.3827+0.2948D in the low dose range of 0～1.0 Gy. The HPRT gene mutation frequencies had better linear relations with chromosome aberrations and micronuclei. The results show that HPRT mutation can be used in the dose estimation of low LET radiation, especially applied to detection of monogenic mutation and assessment of radiation risk.

关 键 词：HPRT基因突变 剂量估算 辐射剂量 辐射防护 体细胞 

分 类 号：R14[医药卫生—公共卫生与预防医学]