阿特拉津降解菌株的分离和鉴定  

ISOLATION AND IDENTIFICATION OF ATRAZINE-DEGRADING STRAINS

作　　者：蔡宝立[1] 黄今勇[1] 石建党[2] 张心平[1] 刘海[1] 朱昌寿[3] 

机构地区：[1]南开大学生命科学学院,天津300071 [2]天津医科大学分析中心 [3]南开大学元素有机化学研究所,天津300071

出　　处：《微生物学通报》2001年第2期22-26,共5页Microbiology China

基　　金：天津市自然科学基金资助项目!(No.993604611)

摘　　要：从农药厂废水中分离到６株能以除草剂阿特拉津为唯一氮源生长的细菌，即假单胞菌（Ｐｓｅｕ－ｄｏｍｏｎａｓ ｓｐｐ．）ＡＤ１、ＡＤ２和 ＡＤ６，土壤杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍ　ｓｐ．）ＡＤ４，黄单胞菌（Ｘａｎｔｈｏｍｏｎａｓ ｓｐ．）ＡＤＳ，欧文氏菌（Ｅｒｗｉｎｉａ ｓｐ．）ＡＤ７。ＡＤ１菌株能使无机盐培养基中的 ０．３ｇ／Ｌ阿特拉津在７２ｈ内降解９９，９％。当以ＡＤ１、ＡＤ２、ＡＤ４、ＡＤ５、ＡＤ６和ＡＤ７菌株的总ＤＮＡ为模板进行ＰＣＲ扩增时，除ＡＤ２菌株以外，均得到了与文献报道的假单胞菌ＡＤＰ菌株的阿特拉津氯水解酶基因（ａｔｚＡ）同源的ＰＣＲ产物。Six atrazine-degrading strains, Pseudomonas spp. AD1, AD2, AD6, Agrobacterium sp. AD4, Xanthomonas ADS, and Erwinia sp. AD7, were isolated from industrial wastewater. These strains are able to grow on atrazine as sole nitrogen source. Strain AD1 is able to degrade atrazine of 0. 3g/L in minimal medium at a percentage of 99. 9% in 72 hours. PCR products that are homologous to the atrazine chlorohydrolase gene atzA)from Pseudomonas sp. strain ADP were obtained by PCR method using total DNA of the strains AD1,AD4,AD5,AD6,and AD7 as templates.

关 键 词：阿特拉津 生物降解 菌株分离 氯水解酶基因 农药 

分 类 号：Q93[生物学—微生物学]