柚品种的等位酶变异研究  

ALLOZYME VARIATION IN POMELO [CITRUS GRANDIS(L.) OSBECK]

作　　者：张太平[1] 彭少麟[2] 王峥峰[2] 凌定厚[2] 

机构地区：[1]华南理工大学造纸与环境工程学院环境科学与工程系,广州510641 [2]中国科学院华南植物研究所,广东广州510650

出　　处：《热带亚热带植物学报》2001年第1期55-62,共8页Journal of Tropical and Subtropical Botany

基　　金：中日美国际合作华南退化坡地绿色食品生产及保护与提高生物多样性研究项目!(199906);中国科学院院地合作项目!(1999年度)

摘　　要：研究了柚的４８个品种的等位酶变异。利用等位酶分析技术对柚的酯酶（ＥＳＴ）、６－磷酸葡萄糖异构酶（ＰＧＩ）、６－磷酸葡萄糖变位酶（ＰＧＭ）、莽草酸脱氢酶（ＳＫＤ）、超氧化物歧化酶（ＳＯＤ）共５个酶系统的１０个等位酶基因座进行了分析．除ＰＧＩ－ｌ、ＰＧＩ－２两个基因座外，其它８个均为多态性基因座；１０个等位酶基因座共观察到的等位基因２５个，平均每个基因座的有效等位基因数目为１．５５，基因多样度０．２８０５。柚的品种间具有较为丰富的等位酶标记遗传多样性，但柚类种质资源群体总的遗传多样性水平偏低。柚的较低的有效等位基因数目与基因多样度可能由于人工选择及资源流失造成。Allozyme variation was examined in 48 pomelo cultivars from the collection nursery of the Chinese Academy of Agricultural Sciences. Ten loci observed in 5 enzyme systems, Esterase (EST), Phosphoglucoisomerase (PGI), Phosphoglucomutase (PGM), Shikimate dehydrogenase (SKD), and Superoxide dismutase (SOD) were encoded by 25 alleles. Except for 2 loci of PGI, all other 8 loci were polymorphic. Effective number of alleles per locus was l.55, and the expected heterozygosity was 0.2805, Which were comparatively low. Pomelo cultivars are rich in allozymic genetic diversity. Lower level of total germplasm diversity is; probably due to a long history of artificial selection and gene loss.

关 键 词：柚子 等位酶 遗传多样性 品种 

分 类 号：Q946.5[生物学—植物学]