从12S rRNA基因序列探讨中国10种壁虎的系统关系  

PHYLOGENY OF TEN SPECIES OF CHINESE GEKKONID LIZARDS (GEKKONIDAE: LACERTILIA) INFERRED FROM 12 S rDAN DNA SEQUENCES

作　　者：韩德民[1] 周开亚[1] 王义权[1] 

机构地区：[1]南京师范大学遗传资源研究所,南京210097

出　　处：《动物学报》2001年第2期139-144,共6页ACTA ZOOLOGICA SINICA

摘　　要：对肌肉样品采用SDS/蛋白酶K裂解、酚 氯仿抽提和乙醇沉淀提取总DNA ,用线粒体 12SrDNA通用引物扩增 ,用银染测序和ABIPrism 310型遗传分析仪测定了Gekkojaponicus,G .swinhonis,G .hokouensis,G .gecko,Cyrtopodionelongatus,C .russowi,Teratoscincusroborowskii,Hemidactylusbowringii,H .frenatus和Gehyramutilata 10种壁虎的线粒体 12SrRNA基因片段的序列。对位排列后的序列长 42 1bp ,含 2 0 1个变异位点。属间核苷酸变异范围为 0 2 2 8～ 0 2 82 ,属内是 0 0 0 5～ 0 2 6 3。重建的分子系统树将 10种壁虎聚为三支 :第1支是大壁虎和截趾虎 ;第 2支由壁虎属其余的 3个种构成一个单系 ;第三支由其余 3个属的 5个种组成。研究结果表明 :截趾虎与壁虎属 4物种亲缘关系较近 ;吐鲁番沙虎和弯脚虎属 2物种、蜥虎属 2物种之间的亲缘关系较近 ,支持Russell (1976 )关于弯脚虎属与蜥虎属间进化关系的研究结果而不支持Kluge (1987)将沙虎属另立亚科的系统发生假说 ;铅山壁虎与无蹼壁虎最先聚为姐妹群 ,再与多疣壁虎相聚组成一个单系。The studies on phylogenetic relationships of gekkonid lizards have been largely based on morphological characters before, including the structure of digits and internal anatomy. In China, the phylogeny among some gekkonid lizards based on karyotype, nucleolar organizer and LDH (lactic dehydrogenase) isozyme was reported. However, nucleotide sequence data have not yet been employed to study the phylogeny of gekkonid lizards. In the present study, phylogenetic relationship among 10 Chinese gekkonid lizard species was inferred based on sequences of 12S rRNA gene fragment. 1. Material and method Total DNA was extracted from about 50 mg of the muscle tissue from 10 gekkonid lizards (Gekko japonicus, G. swinhonis, G. hokouensis, G. gecko, Cyrtopodion elongatus, C. russowi, Teratoscincus roborowskii, Hemidactylus bowringii, H. frenatus and Gehyra mutilata) collected in China. The samples were either frozen (-20℃), or ethanol or formalin preserved (Table 1). Two primers L1091 and H1478 (Kocher et al., 1989) were used for amplification and sequencing. Sequencing were performed manually using silver staining technique or automatically in an ABI Prism 310 Genetic Analyzer. All sequences were aligned with the program of Clustal w (Thompson et al., 1994). The DNA sequences were analyzed with the MEGA program (1 02 edition). For distance estimation, we deleted sites with gaps in each pairwise comparison (Table 2). The phylogenetic trees of NJ, UPGMA and MP were reconstructed with the same program based upon Juke Cantor distance, with bootstrap test (1 000 replications) (Fig.1). 2. Result and discussion The sequences have been deposited in GenBank (accession numbers: AF236819 and AF323510～323518). Aligned sequences of 421 available base pairs (including 201 variant sites) were obtained. The percentage of sequence divergence (sequence difference / (sequence size) among the five genera was 0 228～0 282, within the genera 0 005～0 263. Tricotomous clusters were obtained by those methods i

关 键 词：壁虎科 分子系统发生 12SrRNA基因序列 

分 类 号：Q959[生物学—动物学]