大鼠颌下腺及其离体培养细胞脱氢表雄酮(DHEA)的免疫组织化学定位  

IN VIVO AND IN VITRO THE IMMUNOLOGICAL LOCALIZATION OF DHEA IN RAT SUBMAXILLARY GLAND

作　　者：姚兵[1] 黄威权[1] 张崇理[2] 王江华[1] 

机构地区：[1]第四军医大学组织胚胎学教研室,西安710032 [2]中国科学院动物研究所生殖生物学国家重点实验室,北京100080

出　　处：《动物学报》2001年第2期176-178,T001,共4页ACTA ZOOLOGICA SINICA

摘　　要：用免疫组织化学ABC法 ,研究了颌下腺及无血清培养的颌下腺上皮细胞DHEA的定位。结果显示 ,大鼠颌下腺的浆液性腺泡的上皮细胞及各级导管上皮细胞均呈DHEA免疫反应阳性 ,无血清培养腺上皮细胞也呈DHEA免疫反应阳性 ,阳性物质分布于胞质 ,胞核呈阴性反应。此结果提示 :大鼠颌下腺能自身合成DHEA ,DHEA对消化功能可能具有重要的调节作用。The submaxillary is situated below the floor of the mouth just beneath the body of the mandible. The secretory portion of the gland is composed of tubulo alveolar acini of the mucous and serous types, their secretion contains many enzyme which hydrolyzes the polysaccharide starch into the disaccharides maltose and isomaltose. Previous studies have described that rat submaxillary could not only secret digestive fluid but also synthesize many biological activated substance, such as nerve growth factor(NGF), epithelial growth factor(EGF), retina nodal cell nerve induced factor, 5 HT and GnRH etc. DHEA is the precursor of sexual hormone, this substance and its combination can be situated in the rat brain whose suprarenal or testis is ablated. Recent studies have found that DHEA could also exist in adrenal gland, uterus and testis. However, whether DHEA could exist in submaxillary remains unknown. This study was undertaken to demonstrate the localization of DHEA in submaxillary gland and the epithelial cells from submaxillary gland cultured in serum free medium. The paraffin and culture sections were washed by PBS (pH 7 1, 0.01 mol/L)for five minutes three times, and incubated in methanol H 2O 2 for 20 min to remove endogenous peroxidase and then washed by PBS(pH7 1, 0 01 mol/L) 5 minutes three times. They were then stained according to the immunohistochemical ABC method. Tissue sections were incubated at 4℃ for 24 hr in the primary antibodies of rabbit anti DHEA antibody and rabbit anti keratin antibody(1∶100 dilution), respectively. The secondary antibody, biotin labeled horse anti rabbit IgG(1∶200 dilution) was incubated at room temperature for one hour and ABC complex(1∶100 dilution) incubated at room temperature for 30 min. The negative control tissue sections were incubated with normal rabbit serum and phosphate buffer salt as primary antibodies. The results showed that the epithelial cells cultured in serum free medium exhibited keratin positive reaction, the positive substance was distribu

关 键 词：大鼠 颌下腺 脱氢表雄酮 细胞培养 免疫组织化学 

分 类 号：Q45[生物学—生理学]