应用SDS-PAGE显示小分子多肽技术的探讨  

Study on Analysing Methods of Low Molecular Peptides with SDS-Polyacrylamide Gel Electrophoresis

作　　者：石继红[1] 赵永同[1] 张英起[1] 颜真[1] 韩苇[1] 王俊楼[1] 

机构地区：[1]第四军医大学生物技术中心,西安710032

出　　处：《生物工程进展》2001年第1期38-41,共4页Progress in Biotechnology

摘　　要：为探讨显示小分子多肽技术 ,应用SDS PAGE寻找更简单、快捷的分析小分子多肽的方法。采用 8%T ,3%C的丙烯酰胺浓缩胶和含 6mol/L脲 (或含 2 4 %的甘油 )的 16%T ,6%C的丙烯酰胺分离胶的双层不连续SDS PAGE和三羟基甘氨酸电泳缓冲液显示蛋白分子量标准 ( 2 512～16 94 9kD)和待测样品 ,并对蛋白分子量标准在这两种分离胶中进行了回归分析。结果表明在这两种条件下均能显示分子量小至 2 512kD的多肽 ;多肽样品在含脲和在含甘油的 2L T SDS PAGE中均有较好的显带 ;蛋白分子量标准的直线回归系数分别为r =- 0 966和r =- 0 964。它们是显示小分子多肽和估测其相对分子质量及对多肽分子进行进一步分析的更为简便易行的方法。To study the methods of analysing low molecular polypeptides and to simplify the three layers of tricine(3L\|T)\|sodium dodecyl sulfate\|polyacrylamide gel electrophoresis(SDS\|PAGE),we developed a two layers of tricine(2L\|T)\|SDS\|PAGE.Meanwhile,we also compared the differential function between 6mol/L urea and 24% glycerol in a 16% T,6% C separating arcylamide gel.Molecular weight markers(2\^512～16\^949kD) and samples to be measured formed obvious bands with one layer of a 8% T, 3% C acrylamide stacking gel.Molecular weight markers were analysed by linear regression(lg Mr \|μ) in the above 2L\|T\|SDS\|PAGE.These results suggest that low molecular weight polypeptides can be seen well both in the separating gel with 6mol/L urea and in the separating gel with 24% glycerol by 2L\|T\|SDS\|PAGE.The correlation coefficients of molecular weight markers are -0\^996 in urea and -0\^964 in glycerol.They are conventional,stable,simple to operate and are well established methods for analysis of low molecular weight polypeptides.\;

关 键 词：SDS-PAGE 肽 蛋白质 三羟甘甘氨酸 应用 分析方法 

分 类 号：Q516.03[生物学—生物化学] Q510.3