芳香族化合物生物降解的研究进展  

Advance on the Studies of Biodegradation of Aromatic compounds

作　　者：孙艳[1] 钱世钧[1] 

机构地区：[1]中国科学院微生物所,北京100080

出　　处：《生物工程进展》2001年第1期42-46,共5页Progress in Biotechnology

摘　　要：本文综述了以苯、取代苯、联苯和多环芳烃为代表的芳香族化合物的生物降解途径 ,其共同之处在于经过两步双加氧酶作用 ,生成二醇和开环。两步双加氧酶分别为芳环羟基化双加氧酶和芳环断裂双加氧酶。以甲苯途径为代表讨论了芳香族化合物的分子生物学研究情况。代谢工程研究是九十年代兴起的芳香族化合物生物降解的研究内容 ,通过对甲苯途径的代谢工程研究明确了途径中的关键酶 ,并通过对关键酶的活性提高使整个途径的代谢流增加。Biodegradation of aromatic compounds such as benzene\,alkylbenzenes\,biphenyl and polycyclic aromatic hydrocarbons is reviewed.The conversion of them to cis \|diols and the ring\|cleavage by two kinds of dioxygenases are all involved in the pathway.The molecular biology research about the TOL\|pathway is also discussed here.Metabolic engineering is introduced into the aromatic compounds degradation in early 90's.In the pathway,rate\|controlling enzyme can be determined and the flux through the pathway increased by increasing the titer of the rate\|controlling enzyme.\;

关 键 词：芳香族化合物 生物障碍 双加氧酶 分子生物学 代谢工程 

分 类 号：O625[理学—有机化学] X172[理学—化学]