三种蜘蛛粗毒对NG108-15细胞电压门控钠通道的抑制作用  

Inhibition of Voltage gated Sodium Channel in NG108 15 by the Venoms from Three Kinds of Spider

作　　者：肖玉成[1] 彭宽[1] 陈湘定[1] 梁宋平[1] 

机构地区：[1]湖南师范大学生命科学学院,中国湖南长沙410081

出　　处：《生命科学研究》2001年第1期42-47,共6页Life Science Research

基　　金：国家自然科学基金项目!(397710 5 8)

摘　　要：利用小鼠神经细胞瘤×大鼠神经胶质细胞的杂交细胞 NG1 0 8-1 5,通过全细胞记录 ( whole-cellrecording)模式的膜片钳技术 ,检验了虎纹捕鸟蛛 ( Selenocosmia huwena)、海南捕鸟蛛 ( Selenocosmia hainana)和广西大疣蛛 ( Macrotheleguangxia sp)的粗毒对 NG1 0 8-1 5细胞膜上电压门控 TTX敏感型钠电流和延迟整流钾电流的作用 .结果表明 ,三种蜘蛛粗毒对外向延迟整流钾电流没有明显作用 ,但对 TTX敏感型的快钠电流表现出较强的抑制效应 .抑制效应呈量效关系 .三种粗毒抑制钠电流的 EC5 0 相应约为 :3.4 mg/L;1 .8mg/L和 1 1 .0 mg/L.Using whole cell patch clamp technology, the effects of the venoms from three kinds of spider (Selenocosmia huwena, Selenocosmia hainana and Macrothele guangxia sp) on voltage activated TTX sensitive(TTX S) Na + current and delay rectified K + current of undifferentiated NG108 15 were observed. The results showed that they had no evident effect of outword delay rectified K + current,while they could inhibit TTX S fast Na + current.The inhibitions were dose dependent.The EC 50 values of them were approximate 3.4 mg/L( Selenocosmia huwena ); 1.8 mg/L( Selenocosmia hainana );11.0 mg/L( Macrothele guangxia sp),respectively.

关 键 词：蜘蛛 NG108-15 钠电流 全细胞记录 钠通道阻断活性 蛛毒 

分 类 号：R996.3[医药卫生—毒理学]