微阵列(microarrays)技术及其应用  

Microarray technology and its application

作　　者：杨劲松[1] 陈诗书[1] 

机构地区：[1]上海第二医科大学生化教研室分子生物学实验室人类基因治疗研究中心,上海200025

出　　处：《生命科学》2001年第2期85-88,共4页Chinese Bulletin of Life Sciences

摘　　要：微阵列分为ｃＤＮＡ微阵列和寡聚核苷酸微阵列。微阵列上“印”有大量已知部分序列的ＤＮＡ探针，微阵列技术就是利用分子杂交原理，使同时被比较的标本（用同位素或荧光素标记）与微阵列杂交，通过检测杂交信号强度及数据处理，把他们转化成不同标本中特异基因的丰度，从而全面比较不同标本的基因表达水平的差异。微阵列技术是一种探索基因组功能的有力手段。Microarrays include cDNA microarray and oligonucleotide microarray. A lot of known partial sequences DNA probes were spotted on microarrays.Using principle of molecular hybridization,the compared samples (labelled with fluorescent or radioactivation)were hybridized parallelly with microarrays, then the intensity of hybridized signals were detected and transformed to proficiency of target genes by deta extraction system,the different gene expression of samples were compared in global. The technology is a efficient way to study genomic functions.

关 键 词：微阵列 分子杂交 基因组功能 应用 

分 类 号：Q781[生物学—分子生物学] Q7－34