扶正化瘀中药体外对大鼠纤维肝肝细胞白蛋白合成、分泌的影响  

Effects of Chinese Medicinals with Right-supporting and Phlegm-transforming Actions on Albumin Synthesis and Secretion of Fibrous Hepatic Cells of Rat in Vitro

作　　者：李旭东[1] 王树槐[2] 蒋家道 李登銮[2] 朱海杭[2] 朱瑞锦[2] 王逢民[3] 

机构地区：[1]江苏扬子江药业集团博士后科研工作站,江苏泰州225321 [2]扬州大学医学院附属苏北医院,江苏扬州225001 [3]泰兴市人民医院,江苏泰兴225400

出　　处：《中成药》2001年第4期274-277,共4页Chinese Traditional Patent Medicine

摘　　要：目的 :观察扶正化瘀中药体外对大鼠纤维肝肝细胞白蛋白合成、分泌的影响。方法 :苦杏仁甙 ,丹参水溶性提取物 ,冬虫夏草 ,秋水仙碱作用于体外原代单层培养的大鼠纤维肝肝细胞 ,用ELISA方法检测。结果 :除水仙碱外 ,苦杏仁甙+冬虫夏草 ,冬虫夏草 ,丹参水溶性提取物具有明显提高大鼠纤维肝肝细胞合成、分泌白蛋白的作用 (P <0 .0 5 ) ,以苦杏仁甙 +冬虫夏草组作用最明显。病因是否去除 ,对大鼠纤维肝肝细胞合成、分泌白蛋白功能和药物对其作用有明显影响(P <0 .0 5 - 0 .0 1)。结论 :苦杏仁甙 +冬虫夏草 ,冬虫夏草 ,丹参水溶性提取物能促进大鼠纤维肝肝细胞合成、分泌白蛋白。Objective:To investigate the effects of Chinese Medicinals with right supporting and phlegm transforming actions on albumin synthesis and secretion of fibrous hepatic cells of rat in vitro. Methods: Amygdalin, the water soluble extract of Radix Salviae Miltiorrhizae, lordyceps, and colchicine were used to act on fibrous hepatic cells of rat produced by primary monolayer culture in vitro. The results were determined by ELISA method. Results: Besides colchicine, the others amygdalin and cordyceps. Cordyceps, the water soluble extract of Radix Salviae Miltiorrhizae) showed the obvious raising actions on albumin synthesis and secretion of fibrous hepatic cells of rat ( P <0.05). The action of amygdalin and cordyceps group was the greatest. Whether the etiologic factors had been removed would greatly influence the functions of albumin synthesis and secretion of fibrous hepatic cells of rat and action of Chinese medicinals on the functions ( P <0.05～0.01). Conclusions: Amygdalin and cordyceps, cordyceps, the water soluble extract of Radix Salviae Miltiorrhizae can promote albumin synthesis and secretion of fibrous hepatic cells of rats.

关 键 词：扶正化瘀 肝纤维化 肝细胞 原代单层培养 白蛋白 合成 分泌 中医药治疗 大鼠 

分 类 号：R259.752[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]