脑突触体摄取5-羟色胺研究方法的建立及应用  

Establishment and Use of a Method for Studying Uptake of 5-Hydroxytryptamine (5-HT) by Brain Synaptosomes

作　　者：董文心[1] 李建其[1] 倪湘莲[1] 黄丽瑛[1] 

机构地区：[1]上海医药工业研究院,上海200437

出　　处：《中国医药工业杂志》2001年第3期118-121,共4页Chinese Journal of Pharmaceuticals

摘　　要：建立了稳定可靠的研究脑突触体摄取递质的方法 ,并对芳烷酮哌嗪类 SIPIyy系列新化合物进行了抑制脑突触体对 5 - HT再摄取作用的初步研究。其结果显示 :突触体悬液中的蛋白含量稳定 ,组间变异为 5 .8% ;该系列化合物均有不同程度的抑制脑突触体对 5 - HT再摄取的作用 ,而以 SIPIyy2 4的作用最强 ,有望发展成为新的抗抑郁药。A stable method has been established to study the uptake of 5 HT by brain synaptosomes. The inhibition of re uptake for 5 HT by synaptosomes with SIPIyy series new compounds was studied. The result suggested that content of synaptosomes in extracted suspension was very stable, the coefficient of variation of inter assays was 5.8%. These new compounds had inhibitory effect in different degree for 5 HT uptake by synaptosomes with fluoxetine as the reference standard, among them SIPIyy24 was the most effective.

关 键 词：5-羟色胺 脑突触体 再摄取 氟西汀 SIPIyy24 药物筛选 

分 类 号：R965.1[医药卫生—药理学]