RP-HPLC同时测定利福肼胺胶囊中三组分的含量  

Determination of Three Components in Li-Fu-Jing-An Capsules

作　　者：周学敏[1] 张勤[1] 陈宁[1] 薛玉英[1] 徐斌[1] 

机构地区：[1]江苏省药物研究所,江苏南京210009

出　　处：《中国医药工业杂志》2001年第3期128-130,共3页Chinese Journal of Pharmaceuticals

摘　　要：用 RP- HPL C法测定利福肼胺胶囊中利福平、异烟肼和吡嗪酰胺含量。采用 Delta pak C1 8色谱柱 ,流动相分别为甲醇 - p H 4.5磷酸盐缓冲液 (75∶ 2 5 )和甲醇 - p H 3.5磷酸盐缓冲液 (6∶ 94) ,检测波长 2 5 4nm。方法回收率为99.5 %～ 10 0 .2 % ,RSD均小于 1.6 0 %。Three components in Li Fu Jing An capsules were determined by HPLC. A Waters Delta pak C 18 column was used, with the mobile phase of methanol phosphate buffer (pH 4.5)(75∶25) for rifampicin and methanol phosphate buffer (pH 3.5)(6∶94) for isoniazid and pyrazinamide, at the detection wavelength of 254 nm. The average recoveries were 99.5%~100.2%, RSD s were less than 1.60%.

关 键 词：利福平 异烟肼 吡嗪酰胺 RP-HPLC 测定 利福肼胺胶囊 

分 类 号：R978.3[医药卫生—药品] R927.2[医药卫生—药学]