补肾复方含药血清对骨吸收陷窝影响的时效关系研究  

The Effect of Bushen Fang on Osteoclasts Resorption by Using Drug Sera Method

作　　者：詹红生 石印玉[2] 赵咏芳[2] 谢远军 褚海林 

机构地区：[1]浙江中医学院,杭州310053 [2]上海中医药大学附属曙光医院

出　　处：《浙江中医学院学报》2001年第2期21-22,共2页Journal of Zhejiang College of Traditional Chinese Medicine

基　　金：浙江省自然基金资助项目! (No3990 10 );国家九五攻关项目!(No96 90 6 0 90 5 )

摘　　要：目的　探讨补肾复方用于破骨细胞药效观察的含药血清制备条件之一 (时效关系 )。方法　取体重 2 70± 2 0 g的 SD大鼠 ,雌雄各半 ,每组 10只 ,同等条件下制备补肾复方含药血清和对照血清 ,分别观察不同采血时间、不同喂药时间的含药血清对骨吸收陷窝数量的影响。结果　末次灌胃后 1h采血组的骨吸收陷窝数明显少于其它各组 (P<0 .0 5 ) ;灌胃 1d和 3d、 7d组比较其含药血清的药效无差异 ,而与对照血清差异显著。结论　补肾复方含药血清对破骨细胞骨吸收功能具有明显的抑制作用 ;补肾复方用于破骨细胞药效观察的大鼠含药血清制备条件之一为连续 2次 (间隔 2 h)灌胃、末次灌胃后To evaluate the condition of drug sera on osteoclast(time-effect relationship).Method SD rats,average weight 270±20g,half female and half male,each group has 10 rats.On the same condition collect the effect Bushen Fang sera and control sera,observe different collection time,different administration time,different sera addition on the change in value of resorption lucuna.Result On the condition of 1h after the second administration,the lucuna number was lower than that of other group( P< 0 05).The effects of administration of 1d,3d and 7d had no differences,but had significant difference with that of the control.Conclustion Bushen Fang sera could inhibit osteoclast resorption,and the condition was two administrations(between 2h),1h after the second administration collecting sera.

关 键 词：补肾方 含药血清 时效 破骨细胞 吸收陷害 骨吸收 大鼠 

分 类 号：R274.91[医药卫生—中西医结合] R285.5[医药卫生—中医骨伤科学]