电针对大鼠胃运动、P物质和胃动素的影响  

Effect of electro-acupuncture on gastric motility, substance P(SP) and motilin (MTL) in rats

作　　者：易受乡[1] 林亚平[1] 严洁[1] 常小荣[1] 杨昱[1] 

机构地区：[1]湖南中医学院针灸推拿系,湖南省长沙市410007

出　　处：《世界华人消化杂志》2001年第3期284-287,共4页World Chinese Journal of Digestology

基　　金：国家攀登课题预选项目资助;No.95-预-19-221

摘　　要：目的为了探讨针刺足阳明经穴对胃运动的调整作用是否与脑肠肽有关。方法 60只大鼠随机分为正常对照组、模型组、针剌四白组、天枢组、足三里组、非穴组,以乙醇灌胃造成大鼠胃粘膜损伤模型,气囊法测量胃运动频率和波幅的变化率,采用放免分析法(RIA)检测大鼠胃窦及延髓内SP,MTT含量。结果模型组胃运动频率和波幅呈抑制状态,其胃运动频率和波幅的变化率(％)与对照组比较出现显著差异(-4.7±10.3vs-19.4±17.2.P<0.05;1.8±14.1 vs -51.6±29.0,P<0.01),针剌四白、天枢、足三里穴使胃运动恢复,其波幅(％)较模型组比较分别为-6.5±23.3,1.5±20.1,6.9±25.2vs-51.6±29.0,P<0.01)。针剌非穴组与对照组比较则末出现明显差异。与此同时胃窦、延髓内SP,MTL含量出现相应变化,模型组胃窦SP含量(pg·ML^(-1))低于对照组(41±11vs56±13,P<0.05),针剌天枢、足三里后胃窦SP含量较模型组明显升高(58±13和61±13vs41±11,P<0.05)。模型组延髓SP含量高于正常组,针剌组则均低于模型组。模型组胃窦、延髓内MTL含量(Pg·mL^(-1))均低于对照组,针剌足三里其延髓MTL含量明显高于模型组(59±10vs47±13,P<0.05).三穴比较针剌四白穴主要影响胃窦MTL含量,针刺天枢主要影响胃窦SP含量变化,针剌足三里穴既使胃窦SP升高又使延髓MTL含量升高。结论针剌足阳明经对胃运动的调整作用有脑肠肽参与,但上述三穴对胃运动的影响所涉及的脑肠肽不完全一致。AIM To clarify whether the gut peptides are the substantial elements linking stomach and Foot-Yangming Channel. METHODS Sixty rats were divided into six groups: normal control group, model group (group with gastric mucous damage, GMD), Sibai group (with EA Sibai point +GMD, Tianshu group (with EA Tianshu+GMD), Zusanli group (with EA Zusanli point+GMD), non-point group (with EA non.point+GMD). The model of gastric mucous damage (GMD) was induced by infusing pure Alcohol into gastric cavity. The frequency end amplitude of gastric motillty were monitored by the method of 'Gas Balloon'. Contents of SP and MTL in antrum and medulla were measured by radioimmunoaseay. RESULTS Inhibitory effect of the frequency and amplitude of gastric motility were shown in model group, It's change rate (%) of frequency and amplitude was remarkably different compared with control group (-4.7±13.0 vs -19.4±17.2, P<0.05; 1.8±14.1 vs -51.6±29.0, P<0.01). After needling the points of Sibai, Tienshu, Zusenli, the gastric motility restored to normal. Compared with control group, the amplitude showed significant difference (Sibai -6.5±23.3, Taishu 1.5±20.1, Zusenli 6.9±25.2 vs model -51.6±29.0, P<0.01, respectively). But there was no difference in non-point group, At the same time, Sp and MTL in antrum end medulla varied analogously. Levels (pg·mL^(-1)) of Sp in antrum of model group were lower then that in control group (41±11 vs 56±13, P<0.05). And after needling points of Tainshu, Zusanli, SP levels in antrum were much higher compared with model group (Tainshu 58±13 and Zusenli 61±13 vs model 41±11, P<0.05 respectively). SP levels In medulla in model group were higher then control group, but those in acupuncture groups were lower than that in model group. Antrum and medulla MTL levels (pg·mL^(-1)) in model group were all lower than that in control group. After needling Zusanli, the medulla MTL level was much higher than that in model group (59±10 vs 47±13, P<0.05). The effect of three points was compared, the change of MTL

关 键 词：电针 胃肠活动 针灸效应 P物质 胃泌素类 

分 类 号：R245[医药卫生—针灸推拿学]