丹参对转化生长因子β_1刺激的NIH/3T3细胞表达Ⅰ型胶原和c-fos mRNA的影响  

Effect of Saliva Miltiorrhiza on Transforming Growth Factor β1-Stimulated NIH/3T3 Fibroblast

作　　者：王晓玲[1] 刘平[2] 童普德[1] 谭英姿[2] 钱汝红[1] 胡旭东[1] 蒋文娟[1] 

机构地区：[1]上海中医药大学生物教研室,200032 [2]上海中医药大学肝病研究所

出　　处：《中西医结合肝病杂志》2001年第1期19-20,共2页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：上海市高等学校科学技术自然基金

摘　　要：目的:观察丹参对TGFβ_1刺激的NIH/3T3成纤维细胞功能的影响。方法:将丹参灌胃给正常大鼠,分离含药血清,温育TGFβ_1刺激的成纤维细胞。RT-PCR法检测Ⅰ型胶原和c-fos的基因表达。结果:经TGFβ_1刺激后,细胞Ⅰ型胶原和c-fos的mRNA表达明显增加,丹参含药血清可分别抑制由TGFβ_1引起的细胞Ⅰ型胶原和c-fos的mRNA表达的增加,结论:丹参可能通过抑制c-fos的基因表达进而抑制Ⅰ型胶原的基因表达。Objective: To observe the effects of saliva miltiorrhiza on functions of transforming growth factor β1-stimulated fibrobiast. Methods: Serum of containing saliva miltiorrhiza was collected from rats and TGFβ1-stimulated fibrobiast cells incubated. Reverse transcription-polymerase chain reaction (RT-PCR) method was used to evaluate type Ⅰ collagen and c-fos mRNA expression. Results: After stimulated by TGFβ1, type Ⅰ collagen and c-fos mRNA expression obviously increased in fibrobiast. Drug serum of saliva miltiorrhiza could inhibit the increasing of type Ⅰcollagen and c-fos mRNA expression by TGFβ1-induced. Conclusion: saliva miltiorrhiza may be inhibited typeⅠ collagen mRNA . expression by suppressing c-fos mRNA expression.

关 键 词：丹参 NIH/3T3成纤维细胞 转化生长因子β1 Ⅰ型胶原 C-FOS 中药 药理 

分 类 号：R285[医药卫生—中药学]