儿童原发性肾病综合征与T淋巴细胞亚群紊乱的关系  

T-lymphocyte subsets analysis with flowcytometry in children with primary nephrotic syndrome

作　　者：刘建华[1] 何威逊[1] 

机构地区：[1]上海市儿童医院肾脏科,上海200040

出　　处：《肾脏病与透析肾移植杂志》2001年第2期123-139,共17页Chinese Journal of Nephrology,Dialysis & Transplantation

摘　　要：目的 :探讨T辅助 (Th)和T毒性 (Tc)淋巴细胞亚群紊乱与原发性肾病综合征患儿疾病发生的关系。方法 :运用三色荧光标记法流式细胞术检测 15例复发的原发性肾病患儿外周血Th和Tc细胞亚群百分率 (% ) ,并以 16例缓解期肾病患儿和 10例正常儿童作对照。　 结果 :正常儿童外周血Th1、Th2和Th0或Tc1、Tc2和Tc0细胞百分率分别为 (13 4± 4 36 ) % ,(2 5 3± 1 97) %和 (1 2 5± 0 92 ) %或 (2 6 2± 9 5 7) % ,(1 14± 0 98) %和 (0 42±0 45 ) %。复发的肾病患儿Th1、Th0或Tc1细胞百分率均减低 ,分别为 (1 2 0± 0 91) % (P <0 0 1)、(0 40± 0 39) %(P <0 0 5 )或 (8 6 2± 7 5 2 ) % (P <0 0 5 ) ,Th2细胞百分率增高 ,为 (7 6 5± 4 5 3) % (P <0 0 1) ,而Tc2和Tc0细胞百分率无变化 ,分别为 (1 5 2± 2 0 7) %和 (0 30± 0 31) % (P >0 0 5 )。缓解期肾病患儿Th和Tc细胞亚群百分率均正常 ,Th1、Th2、Th0细胞分别为 (12 0± 4 75 ) % ,(2 87± 2 46 ) %和 (1 31± 0 87) % ;Tc1、Tc2、Tc0细胞分别为 (2 1 6±7 16 ) % ,(0 95± 0 97) %和 (0 37± 0 36 ) % (P >0 0 5 )。Th1/Th2和Tc1/Tc2比值在正常儿童分别为 5 41± 2 77和79 3± 81 5 ,在复发的患儿均明显下降 ,分别为 0 77?Objective:To investigate the changes of T helper(Th)and T cytotoxic(Tc) lymphocyte subsets in children with nephrotic syndrome(PNS)in relapse and remission. Methodology:31 children(3 12 years old)with PNS were involved in this study,including 15 cases in relapse and 16 cases in remission.Samples of 10 healthy children were used as normal control.All the relapsed children with PNS had been given prednison orally.In the 16 children with PNS of remission,7 had previous prednison treatment alone,and the other 9 be treated by cytotoxic agents in addition to prednison.Triple color flow cytometry was applied in analysis of T lymphocytes subsets(Th1,Th2,Th0 and Tc1,Tc2,Tc0). Results:Significant decrement of total T helper cell and significant increment of total T cytotixic cell counts were found both in relaps and remission groups of the children with PNS,as compared with the control group.Significant increment of Th2 cell percentage and significant decrement of Th0 and Tc1 cell percentage were found in PNS children of relapse,as compared with those of the PNS children in remission.No difference was found in the percentage of the subset of T lymphocytes between the in PNS children of remission and the control group.No significant changes in Tc2 and Tc0 subsets was found( P >0 05).The ratio of Th1/Th2 and Tc1/Tc2 reduced significantly in relapsed PNS children as compared with those of PNS children in remission and of the normal control. Conclusion:The predominance of Th2 and Tc2 lymphocytes subsets is associating with the relapse of nephrotic syndrome in PNS children.

关 键 词：原发性肾病综合征 流式细胞术 TH细胞亚群 Tc细胞亚群 儿童 

分 类 号：R726.91[医药卫生—儿科]